Summary of Study ST001881

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001186. The data can be accessed directly via it's Project DOI: 10.21228/M8DD61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001881
Study TitleNMR Predator Cues Target Signaling Pathways in Toxic Algal Metabolome (Non-polar metabolites)
Study Type1H NMR Metabolomics to elucidate signaling pathway
Study SummaryMetabolomics investigation of the phytoplankton Alexandrium minutum with and without copepod cues in order to explore cell signaling involved in toxin induction.
Institute
Georgia Institute of Technology
DepartmentSchool of Biological Sciences, School of Chemistry and Biochemistry, Center for Microbial Dynamics and Infection, Parker H. Petit Institute for Bioengineering and Bioscience
LaboratoryKubanek Lab
Last NameBrown
First NameEmily
Address950 Atlantic Dr Atlanta, Georgia USA 30332
Emailjulia.kubanek@biosci.gatech.edu
Phone404-894-8424
Submit Date2021-07-19
Num Groups2
Total Subjects40
Study CommentsPart 2 of 3. This part includes NMR analysis of non-polar metabolites using oPLSDA. Parts 1 and 3 inlcude NMR analysis of polar metabolites and the corresponding mass spectrometry metabolomics for both polar and non-polar metabolites.
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2021-07-27
Release Version1
Emily Brown Emily Brown
https://dx.doi.org/10.21228/M8DD61
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001951
Collection Summary:Alexandrium minutum cells were collected by vacuum filtration onto GF/F filters and quenched with liquid nitrogen. Frozen cells with filters were stored in foil (previously muffled for 3 h at 450 °C) at -80 °C until extraction.
Collection Protocol ID:Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf
Sample Type:Algae
Collection Method:Filtration and Freeze
Storage Conditions:-80℃
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