Summary of Study ST002065
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001307. The data can be accessed directly via it's Project DOI: 10.21228/M8S124 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002065 |
Study Title | Metabolic impact of anticancer drugs Pd2Spermine and Cisplatin on the nonpolar extracts of brain from healthy mice (part 2) |
Study Type | NMR-based metabolomics |
Study Summary | Platinum (Pt(II)) drugs, e.g. cisplatin (cDDP), are some of the most used chemotherapeutic agents, yet tumor acquired resistance and high toxicity are still current drawbacks. Palladium (Pd(II))-complexes are alternatives due to similar metal coordination and promising cytotoxic properties. Metabolomics can measure the metabolic response of drug-exposed tissues, unveiling insight into drug mechanisms and new markers of drug efficacy/toxicity. The present 1H NMR metabolomics study aims to characterize the in vivo response of the impact of a Pd(II)-complex with polyamine spermine (Pd2Spm), compared to cDDP, on nonpolar metabolism of brain from healthy mice at 1, 12 and 48 h post-injection times. |
Institute | University of Aveiro |
Department | Department of Chemistry and CICECO-Aveiro Institute of Materials |
Laboratory | Metabolomics from Ana M. Gil |
Last Name | Carneiro |
First Name | Tatiana João |
Address | Campus Universitário de Santiago, Aveiro, Aveiro, 3810-193, Portugal |
tatiana.joao@ua.pt | |
Phone | +351 234 370 200 |
Submit Date | 2022-01-10 |
Num Groups | 9 |
Total Subjects | 45 |
Num Females | 45 |
Raw Data Available | Yes |
Analysis Type Detail | NMR |
Release Date | 2022-02-02 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002153 |
Sampleprep Summary: | The frontal cortex of each mice brain was mechanically grounded in liquid nitrogen and samples were extracted using the biphasic methanol/ chloroform/ water (2:2:1) method. Lipophilic phases were recovered and dried. Previously to NMR acquisition, lipophilic extracts were suspended in 650 µL of CDCl3, containing 0.03% tetramethylsilane (TMS). Samples were then homogenized and transferred into 5mm NMR tubes. |
Processing Storage Conditions: | -80℃ |
Extraction Method: | Biphasic method (methanol/ chloroform/ water) |
Extract Storage: | -80℃ |