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MB Sample ID: SA002217
| Local Sample ID: | C-007 |
| Subject ID: | SU000069 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Infant |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000069 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Infant |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C-007 | SA002217 | FL000668 | high | As Exposure |
Collection:
| Collection ID: | CO000052 |
| Collection Summary: | Fetal cord blood serum was collected immediately after delivery from a saline cleaned umbilical cord using an anticoagulant-free vacutainer tube, after clot formation the tube was centrifuged at 1200 rpm and the serum was collected and stored at -70°C. |
| Sample Type: | Cord Blood Serum |
| Collection Location: | Gómez Palacio, Durango, Mexico |
Treatment:
| Treatment ID: | TR000070 |
Sample Preparation:
| Sampleprep ID: | SP000065 |
| Sampleprep Summary: | Fifty (50) µl of 0.9% NaCl solution containing 4mM sodium formate (Chemical Shift Indicator) and 0.2% NaN3 (to inhibit bacterial growth) in D2O was pipetted into each of the 50 serum samples (150 µl aliquots). Each 200 µl sample was vortexed for 30 seconds and transferred into 3mm NMR tubes (Bruker Bio-Spin, Germany). Pooled serum samples for each phenotype were made by combining 12 µl aliquots from each of the study samples in the phenotypic group (low and high) into two separate eppendorf tubes. A combined total pooled sample was also prepared by combining 6 µl aliquots from all samples into an eppendorf tube. The pooled samples were prepared as described above and were used as quality check (QC) samples. |
| Sampleprep Protocol Filename: | Fetal_Metabolomics_of_iAs_exposure_Metabolomics_Procedure.docx |
| Processing Method: | Dilution with D2O containing 0.9% NaCl and 4 mM Formate |
| Processing Storage Conditions: | On ice |
Analysis:
| MB Sample ID: | SA002217 |
| Analysis ID: | AN000087 |
| Laboratory Name: | RTI, DHMRI |
| Analysis Type: | NMR |
| Acquisition Date: | 41367 |
| Software Version: | TopSpin 3.0 |
| Operator Name: | Wimal Pathmasiri, Kevin Knagge |
| Randomization Order: | Yes |
| Detector Type: | NMR |
| Chromatography ID: | CH000056 |
| Num Factors: | 5 |
| Num Metabolites: | 29 |
NMR:
| NMR ID: | NM000024 |
| Analysis ID: | AN000087 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 1 mM |
| Spectrometer Frequency: | 950 MHz |
| NMR Probe: | 5 mm Inverse ATMA |
| NMR Solvent: | D2O |
| NMR Tube Size: | 3 mm, 4 inches |
| Shimming Method: | Topshim |
| Pulse Sequence: | cpmgpr1d |
| Water Suppression: | presat |
| Pulse Width: | 8.06 s |
| Power Level: | 12.589W |
| Receiver Gain: | 4 |
| Offset Frequency: | 4469.49 Hz |
| Chemical Shift Ref Cpd: | Formate |
| Temperature: | 298 K |
| Number Of Scans: | 256 |
| Dummy Scans: | 16 |
| Relaxation Delay: | 2 s |
| Spectral Width: | 19531.25 Hz |
| Num Data Points Acquired: | 65523 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 |
| Zero Filling: | Yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | Formate |
