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MB Sample ID: SA002945
| Local Sample ID: | F_Dirty_1736 |
| Subject ID: | SU000075 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C3H/HeN |
| Gender: | Female |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000075 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C3H/HeN |
| Gender: | Female |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| F_Dirty_1736 | SA002945 | FL000839 | Dirty Corncob Bedding | Bedding type |
| F_Dirty_1736 | SA002945 | FL000839 | Alum + Ova | Ovalbumin Immunization |
| F_Dirty_1736 | SA002945 | FL000839 | Yes | Peanut Sensitization |
Collection:
| Collection ID: | CO000058 |
| Collection Summary: | - |
| Sample Type: | feces |
Treatment:
| Treatment ID: | TR000076 |
Sample Preparation:
| Sampleprep ID: | SP000071 |
| Sampleprep Summary: | Frozen fecal samples were transferred into numbered homogenizer bead tubes (ceramic) and diluted with D2O (Sigma-Aldrich, St. Louis, MO). Specimens with a mass of less than 100 mg were diluted with 0.5 mL; specimens with a mass of greater than 100 mg were diluted with 1.0 mL. The samples were then homogenized using the MagNA Lyser bead homogenizer for two 30 second pulses at 2000 rpm and then vortexed for an additional 30 seconds. The samples were pelleted by centrifugation at 12000 rcf for 5 minutes. 50 mg of extract were utilized for each specimen, and combined the fecal extract and D2O were a total volume of 630 µL. 70 µL of Chenomx ISTD solution into all tubes. Chenomx ISTD (Chenomx, Edmonton, Alberta, Canada) contains 5mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS, chemical shift indicator), 100 mM imidazole (pH indicator), and 0.2% NaN3 (to inhibit bacterial growth) in D2O. 600 µL was transferred into each NMR tube for analysis. |
| Sampleprep Protocol Filename: | RTI_Food_Allergy_Envirnomental_Metabolomics_Procedure.docx |
Analysis:
| MB Sample ID: | SA002945 |
| Analysis ID: | AN000094 |
| Laboratory Name: | RTI/DHMRI |
| Analysis Type: | NMR |
| Acquisition Date: | 41724 |
| Software Version: | Topspin/ 3.0 |
| Operator Name: | Andrew Novokhatny, Kevin Knagge |
| Randomization Order: | Yes |
| Detector Type: | NMR |
| Data Format: | fid, 1r |
| Chromatography ID: | CH000062 |
| Num Factors: | 15 |
NMR:
| NMR ID: | NM000026 |
| Analysis ID: | AN000094 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| NMR Comments: | Intellegent binning with 50% looseness |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 0.5mM |
| Spectrometer Frequency: | 700 MHz |
| NMR Probe: | cryogenically cooled ATMA inverse probe |
| NMR Solvent: | 10 % D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Gradient |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | yes |
| Pulse Width: | 10.7 microseconds |
| Power Level: | 25.704 W |
| Receiver Gain: | 4.5 |
| Offset Frequency: | 3296.0 Hz |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 25 degrees celsius |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.324 seconds |
| Relaxation Delay: | 2 s |
| Spectral Width: | 20.13580048 |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | DSS |
