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MB Sample ID: SA003073
| Local Sample ID: | 140111dlvsa23_1 |
| Subject ID: | SU000077 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000077 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 140111dlvsa23_1 | SA003073 | FL000871 | breast cancer cells-MDA-MB-468 | Organ |
| 140111dlvsa23_1 | SA003073 | FL000871 | Breast Cancer Cell Culture Pellets | species |
| 140111dlvsa23_1 | SA003073 | FL000871 | Group 7: Homocysteine Treated- 48 hours - Group 7 | Treatment |
Collection:
| Collection ID: | CO000060 |
| Collection Summary: | - |
| Sample Type: | Breast |
Treatment:
| Treatment ID: | TR000078 |
Sample Preparation:
| Sampleprep ID: | SP000073 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Protocol_for_primary_metabolites.docx |
| Sample Spiking: | FAMEs |
Combined analysis:
| Analysis ID | AN000096 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Restek Corporation Rtx-5Sil MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco GC-TOF |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000064 |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
| Instrument Name: | Agilent 6890N |
| Column Name: | Restek Corporation Rtx-5Sil MS |
| Column Pressure: | 7.7 PSI (initial condition) |
| Column Temperature: | 50 - 330C |
| Flow Rate: | 1 ml/min |
| Injection Temperature: | 50C ramped to 250C by 12C/s |
| Sample Injection: | 0.5l |
| Oven Temperature: | 50C for 1 min, then ramped at 20C/min to 330C, held constant for 5 min |
| Transferline Temperature: | 230C |
| Washing Buffer: | Ethyl Acetate |
| Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
| Randomization Order: | Excel generated |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000072 |
| Analysis ID: | AN000096 |
| Instrument Name: | Leco GC-TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | 70eV |
| Mass Accuracy: | Nominal |
| Source Temperature: | 250°C |
| Scanning Range: | 80-500 Da |
| Acquisition Parameters File: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
| Processing Parameters File: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
