Return to study ST000101 main page
MB Sample ID: SA005568
| Local Sample ID: | Mixture B5 |
| Subject ID: | SU000120 |
| Subject Type: | Synthetic Sample |
| Subject Species: | - |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000120 |
| Subject Type: | Synthetic Sample |
| Subject Species: | - |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Mixture B5 | SA005568 | FL001388 | (Arg,Nicotinamide,Lys,Trp,Carnitine)-High ( Met,Pro,Glu,Glucose,Ile)-Low | Mixture Concentration (mM) |
Collection:
| Collection ID: | CO000103 |
| Collection Summary: | - |
| Sample Type: | Synthetic Mixture |
| Volumeoramount Collected: | 50 microliters |
| Storage Conditions: | -80 degree Celsius |
Treatment:
| Treatment ID: | TR000121 |
Sample Preparation:
| Sampleprep ID: | SP000116 |
| Sampleprep Summary: | Two groups of mixtures with 5 replicates each were each made using 20 synthetic metabolites ranging in concentration from 1 to 5 mM. The first ten metabolites were at equal concentrations in both groups, and the remaining 10 metabolites differed between groups with half higher in group A, and half higher in group B. Samples were brought to 50 microliters using 99% D2O and 40 microliters were pipetted into a 1.5 mm tube. |
| Sampleprep Protocol Filename: | SyntheticMix_Concentrations.pdf |
| Sampleprep Protocol Comments: | 10 metabolites were of similar concentration between the two groups. |
Analysis:
| MB Sample ID: | SA005568 |
| Analysis ID: | AN000168 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Analysis Comments: | 1D 1H |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Data Format: | Varian |
| Chromatography ID: | CH000115 |
| Num Factors: | 2 |
| MB Sample ID: | SA005568 |
| Analysis ID: | AN000167 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Analysis Comments: | 1D 13C |
| Software Version: | VNMRJ |
| Operator Name: | Chaevien Clendinen |
| Data Format: | Varian |
| Chromatography ID: | CH000115 |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000034 |
| Analysis ID: | AN000167 |
| Instrument Name: | Varian |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 13C |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 1.5 mm HTS cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Shimming Method: | Manual |
| Pulse Sequence: | 1D 13C |
| Pulse Width: | 60 |
| Receiver Gain: | 28 |
| Temperature: | 22 degree Celsius |
| Number Of Scans: | 8192 |
| Dummy Scans: | 16 |
| Acquisition Time: | 0.8s |
| Relaxation Delay: | 0.1 |
| Spectral Width: | 212 ppm |
| Num Data Points Acquired: | 51282 |
| Real Data Points: | 25641 |
| Line Broadening: | 2 Hz |
| Zero Filling: | 2x |
| Apodization: | Exponential |
| NMR ID: | NM000035 |
| Analysis ID: | AN000168 |
| Instrument Name: | Varian |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 1.5 mm HTS cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 1.5 mm |
| Shimming Method: | Manual |
| Pulse Sequence: | 1D 1H |
| Water Suppression: | Presaturation |
| Pulse Width: | 45 |
| Receiver Gain: | 28 |
| Offset Frequency: | 4.77 |
| Chemical Shift Ref Cpd: | TSP |
| Temperature: | 22 degree Celsius |
| Number Of Scans: | 64 |
| Dummy Scans: | 16 |
| Acquisition Time: | 2.0s |
| Relaxation Delay: | 2s |
| Spectral Width: | 12 ppm |
| Num Data Points Acquired: | 32768 |
| Real Data Points: | 16384 |
| Zero Filling: | 2x |
| Apodization: | Cosine squared |
