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MB Sample ID: SA007942
| Local Sample ID: | amm_form |
| Subject ID: | SU000159 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC - Supplier |
| Subject Comments: | P?+10 |
| Cell Primary Immortalized: | immortalized |
| Cell Passage Number: | P?+10 |
| Cell Counts: | P?+10 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000159 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC - Supplier |
| Subject Comments: | P?+10 |
| Cell Primary Immortalized: | immortalized |
| Cell Passage Number: | P?+10 |
| Cell Counts: | P?+10 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| amm_form | SA007942 | FL001927 | 0.3% ammonium formate | rinsing_solution |
Collection:
| Collection ID: | CO000144 |
| Collection Summary: | - |
| Sample Type: | Jurkat cells, Clone E6-1 |
| Collection Method: | Centrifugation |
| Collection Frequency: | 1200 rpm |
| Collection Duration: | 5 min. |
| Storage Conditions: | -80°C |
| Tissue Cell Quantity Taken: | 1e6 cells |
Treatment:
| Treatment ID: | TR000162 |
| Cell Storage: | Liquid Nitrogen, 5% DMSO |
| Cell Growth Container: | 10 cm2 culture dish |
| Cell Media: | RPMI 1640 |
| Cell Envir Cond: | 95% humidity, 5% CO2 |
Sample Preparation:
| Sampleprep ID: | SP000157 |
| Sampleprep Summary: | 80% MeOH metabolite extraction |
| Sampleprep Protocol ID: | SOP_PP_02 |
| Sampleprep Protocol Filename: | SOP_PP_02_CZU.pdf |
| Processing Method: | cell lysis |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 80% MeOH |
| Extract Concentration Dilution: | 1e6 cells/ 2 mL of extraction solvent |
| Sample Resuspension: | 30 μL 0.1% formic acid in water |
| Sample Spiking: | internal standards: (1) L-tryptophan-2,3,3-d3; (2) creatine-d3; (3) d-leucine-d10; (4) caffeine-d3 |
| Cell Type: | Jurkat, Clone E6-1 |
Combined analysis:
| Analysis ID | AN000222 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000154 |
| Chromatography Summary: | Reversed phase |
| Methods Filename: | PFP-metabolomics-pos-350 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Column Pressure: | 800 bar (max) |
| Column Temperature: | 30 C |
| Flow Rate: | 0.35 mL/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 5 μL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 18 min |
| Randomization Order: | 3,5,7,2,12,10,15,9,13,14,4,8,6,1,11 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000173 |
| Analysis ID: | AN000222 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 320 |
| Dry Gas Flow: | 45 |
| Mass Accuracy: | <2 ppm |
| Spray Voltage: | 3.5 kV |
| Dataformat: | .raw |
| Scan Range Moverz: | 70-700 |
| Skimmer Voltage: | 15 V |
| Acquisition Parameters File: | QE1_CZU_17_SEQUENCE |
