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MB Sample ID: SA008089
| Local Sample ID: | A549_Ctl_2_unL_plate |
| Subject ID: | SU000167 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | A549 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000167 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | A549 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| A549_Ctl_2_unL_plate | SA008089 | FL001966 | unlabeled | Treatment |
Collection:
| Collection ID: | CO000153 |
| Collection Summary: | - |
| Collection Protocol Filename: | WS_Fan_Quench_Cell_Tissue.pdf |
| Collection Protocol Comments: | Collection and quench of cells in tissue culture |
| Sample Type: | Epithelial cells |
Treatment:
| Treatment ID: | TR000172 |
| Treatment Summary: | unlabeled | 13C-Glc |
| Treatment Protocol Filename: | WS_Fan_Cell_Tracer_Ex.pdf |
| Treatment Protocol Comments: | cell culture in unlabeled media for 24h with QDA derivatives | cell culture in 13C-Glc-labeled media for 24h with QDA derivatives |
Sample Preparation:
| Sampleprep ID: | SP000167 |
| Sampleprep Summary: | polar extraction | protein extraction | lipid extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf_ _WS_Fan_Prot_Quant.pdf_ _WS_Fan_Extract_Polar_Lipid_Prot.pdf |
| Sampleprep Protocol Comments: | Polar extraction from cells | Protein extraction and quantification | Lipid extraction from cells |
Combined analysis:
| Analysis ID | AN000235 |
|---|---|
| Analysis type | MS |
| Chromatography type | |
| Chromatography system | |
| Column | |
| MS Type | ESI |
| MS instrument type | FT-ICR-MS |
| MS instrument name | Bruker SolariX FT-ICR-MS |
| Ion Mode | POSITIVE |
| Units | normalized corrected Peak area |
Chromatography:
| Chromatography ID: | CH000164 |
MS:
| MS ID: | MS000186 |
| Analysis ID: | AN000235 |
| Instrument Name: | Bruker SolariX FT-ICR-MS |
| Instrument Type: | FT-ICR-MS |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
