Return to study ST000166 main page
MB Sample ID: SA009071
| Local Sample ID: | 3_1 |
| Subject ID: | SU000185 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000185 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 3_1 | SA009071 | FL002139 | HF | Group |
| 3_1 | SA009071 | FL002139 | Res | State |
Collection:
| Collection ID: | CO000171 |
| Collection Summary: | Blood samples were taken from a peripheral vein. These blood samples (5 mL) were collected into EDTA tubes containing 100 ?L of injected stop solution (dipyrimidole,10 ?M; EHNA, 5 ?M; iodotubercidin, 2 ?M; AOPCP, 50 ?M, in 0.9% NaCl) to reduce blood nucleotide metabolism; stored on ice; and then centrifuged at 3,200 rpm for 10 minutes at 4°C. Plasma aliquots were stored at ?80°C until analyses. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000191 |
| Treatment Summary: | Heart failure|3-month follow up after cardiac resynchronization therapy (CRT)|Controls |
| Treatment Protocol Comments: | All HF patients underwent a baseline evaluation before CRT, including assessment of New York Heart Association (NYHA) functional class, concomitant cardiovascular conditions (e.g., hypertension, coronary artery disease, and diabetes mellitus), electrocardiographic QRS duration and morphologic characteristics, and transthoracic echocardiography. Echocardiographic parameters included LV end-systolic and diastolic dimensions, LVEF, and pulmonary artery systolic pressure. Medication use was recorded and confirmed that patients were taking optimal medication dose for HF. Patients continued on stable medication dosage during the study.|The HF patients returned for a clinical follow-up 3 months after CRT. The NYHA functional class was reassessed, and echocardiography was repeated.|Ten age-matched control patients underwent catheter ablation for supraventricular arrhythmia with a normal left ventricular ejection fraction (LVEF) of greater than 55%. |
Sample Preparation:
| Sampleprep ID: | SP000185 |
| Sampleprep Summary: | For gas chromatographymass spectrometry (GC-MS), plasma (100 ?L) was extracted using a 900-?L methanol:water (8:1, v/v) mixture containing 5 ?g internal standard, myristic-d27 acid, at ambient temperature 33. Supernatant (900 ?L) was transferred and completely dried in a vacuum concentrator. Subsequently, the tubes were methoximated and derivatized and then analyzed using an Agilent 6890 GC oven with Agilent 5973 MS 34. Sample Treatment and Instrumental Conditions for 1H NMR Metabolomic Analysis For nuclear magnetic resonance (NMR) imaging, plasma (60 ?L) was diluted 140 ?L with 0.2M phosphate buffer (pH 7.4):D2O containing a mixture of 16 mM formate and 4 mM TSP (1:1, v/v). After filtering (0.22 ?m), the samples were transferred into a 3-mm-diameter NMR tube. High-resolution 1H NMR spectra were acquired at 600 MHz on a Bruker Avance III 600 spectrometer. Spectra peaks were identified according to Chenomx NMR Suite 6.1 software and data in the literature 35, 36. |
| Sampleprep Protocol Filename: | Methods-For-Upload.docx |
Combined analysis:
| Analysis ID | AN000259 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 GC |
| Column | |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000183 |
| Chromatography Summary: | For gas chromatographymass spectrometry (GC-MS), plasma (100 ?L) was extracted using a 900-?L methanol:water (8:1, v/v) mixture containing 5 ?g internal standard, myristic-d27 acid, at ambient temperature 33. Supernatant (900 ?L) was transferred and completely dried in a vacuum concentrator. Subsequently, the tubes were methoximated and derivatized and then analyzed using an Agilent 6890 GC oven with Agilent 5973 MS 34. Sample Treatment and Instrumental Conditions for 1H NMR Metabolomic Analysis For nuclear magnetic resonance (NMR) imaging, plasma (60 ?L) was diluted 140 ?L with 0.2M phosphate buffer (pH 7.4):D2O containing a mixture of 16 mM formate and 4 mM TSP (1:1, v/v). After filtering (0.22 ?m), the samples were transferred into a 3-mm-diameter NMR tube. High-resolution 1H NMR spectra were acquired at 600 MHz on a Bruker Avance III 600 spectrometer. Spectra peaks were identified according to Chenomx NMR Suite 6.1 software and data in the literature 35, 36. |
| Instrument Name: | Agilent 6890 GC |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000209 |
| Analysis ID: | AN000259 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
