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MB Sample ID: SA011074
Local Sample ID: | FemaleRat3 |
Subject ID: | SU000257 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley rat |
Weight Or Weight Range: | 220-250g |
Gender: | Male/Female |
Animal Housing: | pathogen-free animal facility |
Animal Light Cycle: | 12h light/dark cycle |
Animal Feed: | free access to standard food |
Animal Water: | free access to water |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000257 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley rat |
Weight Or Weight Range: | 220-250g |
Gender: | Male/Female |
Animal Housing: | pathogen-free animal facility |
Animal Light Cycle: | 12h light/dark cycle |
Animal Feed: | free access to standard food |
Animal Water: | free access to water |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
FemaleRat3 | SA011074 | FL002699 | Female | Gender |
Collection:
Collection ID: | CO000247 |
Collection Summary: | Blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum |
Collection Protocol Filename: | 1_COL_PROT.pdf |
Sample Type: | Serum |
Collection Method: | Blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum |
Collection Location: | Lab |
Collection Frequency: | Once |
Collection Duration: | About 10~60 seconds |
Volumeoramount Collected: | 3-5ml |
Storage Conditions: | -80°C |
Collection Vials: | 15ml Polypropylene tube |
Storage Vials: | 1.5ml Eppendorf vial |
Collection Tube Temp: | Room tempreture |
Blood Serum Or Plasma: | Serum |
Treatment:
Treatment ID: | TR000267 |
Treatment Summary: | On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. Then a blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum. All urine and serum samples were frozen at -80°C prior to analysis. |
Treatment: | Intervention |
Treatment Route: | Stay in metabolic cage for 24h urine collection |
Animal Anesthesia: | Anesthesia using Pentobarbital Sodium |
Animal Acclimation Duration: | ?3 days |
Animal Fasting: | 24h |
Animal Endp Euthanasia: | Died in anesthesia status |
Animal Endp Tissue Coll List: | Blood |
Animal Endp Tissue Proc Method: | Centrifuged to obtain serum |
Animal Endp Clinical Signs: | Died |
Sample Preparation:
Sampleprep ID: | SP000261 |
Sampleprep Summary: | Amygdalin was dissolved in acetonitrile-water(1:1) to form 0.1mg/ml solution. Serum samples were unfrozen at room temperature. 500?l of serum sample was mixed with 20?l Amygdalin solution and 100?l 20% Trichloroacetic acid(TCA) solution. After centrifugation at about 14000rpm and -4?, the supernatant was injected into UPLC-TOF MS system. |
Sampleprep Protocol Filename: | 2_PRE_PROT.pdf |
Processing Method: | Precipitation |
Processing Storage Conditions: | Precipitation at room tempreture; centrifugation at -4? |
Extraction Method: | Precipitation by TCA |
Extract Concentration Dilution: | 500?l of serum/620?l |
Extract Storage: | 4? after centrifugation and during analysis |
Sample Spiking: | 20?l Amygdalin solution/620?l |
Combined analysis:
Analysis ID | AN000363 | AN000364 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt-HDMS | Waters Synapt-HDMS |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Height | Peak Height |
Chromatography:
Chromatography ID: | CH000265 |
Chromatography Summary: | Untargeted UPLC-TOFMS |
Methods Filename: | 3_CHR_PROT.pdf |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 24? |
Flow Gradient: | Isocratic 2% B (01 min), linear gradient from 2% to 5% B (12 min), 5% to 12% B (25 min), 12% to 20% B (510 min), 20% to 30% B (1012 min), 30% to 50% B (1213 min), 50% to 100% B (1315 min), isocratic 100% B for 1min, then back to 2% B in 1 min and isocratic 2% B for 3min before next run |
Flow Rate: | 0.5 ml/min |
Injection Temperature: | 24? |
Internal Standard: | Amygdalin |
Sample Injection: | 8?L |
Sampling Cone: | 40V |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 20min |
Capillary Voltage: | Positive ion mode:3kV; Negative ion mode: 2.9kV |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000309 |
Analysis ID: | AN000363 |
Instrument Name: | Waters Synapt-HDMS |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3kV |
Collision Energy: | 6V (trap)/4V (transfer) |
Collision Gas: | Ar |
Dry Gas Flow: | 900 L/h |
Dry Gas Temp: | 450? |
Ion Source Temperature: | 100? |
Ionization: | ESI |
Source Temperature: | 100? |
Desolvation Gas Flow: | 900 L/h |
Desolvation Temperature: | 450? |
Acquisition Parameters File: | Inlet_Method_File_Name:_20100505 _Positive_MS_Mehod_File_Name:_20120221_pos_50-1500 |
Processing Parameters File: | TR_0-18_50-1500_005_020_YES6_YES.mlm |
MS ID: | MS000310 |
Analysis ID: | AN000364 |
Instrument Name: | Waters Synapt-HDMS |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 2.9kV |
Collision Energy: | 6V (trap)/4V (transfer) |
Collision Gas: | Ar |
Dry Gas Flow: | 900 L/h |
Dry Gas Temp: | 450? |
Ion Source Temperature: | 100? |
Ionization: | ESI |
Source Temperature: | 100? |
Desolvation Gas Flow: | 900 L/h |
Desolvation Temperature: | 450? |
Acquisition Parameters File: | Negative_MS_Mehod_File_Name:_20120221_neg_50-1500 |
Processing Parameters File: | TR_0-18_50-1500_005_020_YES6_YES.mlm |