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MB Sample ID: SA011537
| Local Sample ID: | KD_385113_2 |
| Subject ID: | SU000272 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Wistar |
| Gender: | Male |
| Animal Animal Supplier: | Charles River Laboratories |
| Animal Housing: | Division of Veterenary Resources, University of Miami |
| Animal Light Cycle: | 12 hour day / light cycle |
| Animal Feed: | Ad libitum |
| Animal Water: | Ad libitum |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000272 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Wistar |
| Gender: | Male |
| Animal Animal Supplier: | Charles River Laboratories |
| Animal Housing: | Division of Veterenary Resources, University of Miami |
| Animal Light Cycle: | 12 hour day / light cycle |
| Animal Feed: | Ad libitum |
| Animal Water: | Ad libitum |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| KD_385113_2 | SA011537 | FL002873 | ITD+RH | Treatment |
| KD_385113_2 | SA011537 | FL002873 | Hypothalamus | sample type |
Collection:
| Collection ID: | CO000263 |
| Collection Summary: | Rats were euthanized under isoflurane anesthesia (30% O2 / 70% N2O) |
| Sample Type: | Tissue |
| Collection Method: | Hippocampus and Hypothalamus were disected out qucikly and were frozen in liquid nitrogen. |
| Volumeoramount Collected: | Entire hippocampus and hypothalamus were collected. |
| Storage Conditions: | Samples were stored at - 80 o C freezer and shipped to Metabolomics facility on dry ice. |
| Collection Vials: | 1.5 ml tubes |
| Storage Vials: | 1.5 ml tubes |
| Collection Tube Temp: | RT at the time of sample collection |
| Tissue Cell Identification: | Hippocampus and hypothalamus |
| Tissue Cell Quantity Taken: | Entire hippocampus and hypothalamus were collected. |
Treatment:
| Treatment ID: | TR000283 |
| Treatment Summary: | Two groups (i) Insulin-treated diabetic + Recurrent hypoglycemia, (ii) Insulin-treated diabetic + Recurrent hypoglycemia (only insulin injection without hypoglycemia) + Glucose infusion to maintain glucose to baseline levels. |
Sample Preparation:
| Sampleprep ID: | SP000277 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Brain_Tissue_Sampleprep.pdf |
| Sampleprep Protocol Comments: | Appendix C_Protein_DNA_quant.pdf |
| Organ: | Brain |
Combined analysis:
| Analysis ID | AN000398 | AN000399 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak Area |
Chromatography:
| Chromatography ID: | CH000282 |
| Methods Filename: | Metabolomics_LCMSProtocol.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000342 |
| Analysis ID: | AN000398 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000343 |
| Analysis ID: | AN000399 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
