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MB Sample ID: SA011632
| Local Sample ID: | BP295 |
| Subject ID: | SU000275 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Gestational age <37 weeks |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000275 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | Gestational age <37 weeks |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| BP295 | SA011632 | FL002908 | PH (pulmonary hypertension) | Treatment |
Collection:
| Collection ID: | CO000267 |
| Collection Summary: | - |
| Collection Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
| Sample Type: | Umbilical cord plasma |
| Storage Conditions: | - 80°C |
| Storage Vials: | Eppendorf tubes |
Treatment:
| Treatment ID: | TR000287 |
| Treatment Summary: | Nested case-control study |
| Treatment Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
Sample Preparation:
| Sampleprep ID: | SP000281 |
| Sampleprep Summary: | Plasma was added directly to a Waters Ostro plate, mixed with the surrogates, antioxidants, and acetonitrile and the extract eluted through. The extracts were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal standards 1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea and filtered at 0.1 µm prior to analysis. |
| Sampleprep Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
| Extraction Method: | Ostro plate |
| Extract Cleanup: | Ostro plate |
| Extract Storage: | - 20 °C |
| Sample Resuspension: | 100 µL |
| Sample Spiking: | See sample prep protocol file |
| Organ: | Umbilical cord plasma |
Combined analysis:
| Analysis ID | AN000403 |
|---|---|
| Analysis type | MS |
| Chromatography type | |
| Chromatography system | Waters Acquity |
| Column | |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex API 4000 QTrap |
| Ion Mode | NEGATIVE |
| Units | % Area Ratio |
Chromatography:
| Chromatography ID: | CH000285 |
| Chromatography Summary: | Oxylipin analysis |
| Methods Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
| Instrument Name: | Waters Acquity |
| Column Temperature: | 60 C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 16 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
MS:
| MS ID: | MS000347 |
| Analysis ID: | AN000403 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | See protocol file |
| Ion Mode: | NEGATIVE |
