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MB Sample ID: SA011645
Local Sample ID: | BP253 |
Subject ID: | SU000275 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Gestational age <37 weeks |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000275 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Gestational age <37 weeks |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
BP253 | SA011645 | FL002909 | no PH (no pulmonary hypertension) | Treatment |
Collection:
Collection ID: | CO000267 |
Collection Summary: | - |
Collection Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
Sample Type: | Umbilical cord plasma |
Storage Conditions: | - 80°C |
Storage Vials: | Eppendorf tubes |
Treatment:
Treatment ID: | TR000287 |
Treatment Summary: | Nested case-control study |
Treatment Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
Sample Preparation:
Sampleprep ID: | SP000281 |
Sampleprep Summary: | Plasma was added directly to a Waters Ostro plate, mixed with the surrogates, antioxidants, and acetonitrile and the extract eluted through. The extracts were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal standards 1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea and filtered at 0.1 µm prior to analysis. |
Sampleprep Protocol Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
Extraction Method: | Ostro plate |
Extract Cleanup: | Ostro plate |
Extract Storage: | - 20 °C |
Sample Resuspension: | 100 µL |
Sample Spiking: | See sample prep protocol file |
Organ: | Umbilical cord plasma |
Combined analysis:
Analysis ID | AN000403 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | Waters Acquity |
Column | |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap |
Ion Mode | NEGATIVE |
Units | % Area Ratio |
Chromatography:
Chromatography ID: | CH000285 |
Chromatography Summary: | Oxylipin analysis |
Methods Filename: | NewmanLab-FreeOxys-Protocol-P.pdf |
Instrument Name: | Waters Acquity |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 16 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
MS:
MS ID: | MS000347 |
Analysis ID: | AN000403 |
Instrument Name: | ABI Sciex API 4000 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol file |
Ion Mode: | NEGATIVE |