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MB Sample ID: SA011725
| Local Sample ID: | LF liv 3 |
| Subject ID: | SU000277 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 30 weeks |
| Gender: | Female |
| Animal Housing: | Singly housed |
| Animal Feed: | High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet (D12450, Research Diets, New Brunswick, NJ) |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000277 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 30 weeks |
| Gender: | Female |
| Animal Housing: | Singly housed |
| Animal Feed: | High fat diet (D12492, Research Diets, New Brunswick, NJ), Low fat diet (D12450, Research Diets, New Brunswick, NJ) |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| LF liv 3 | SA011725 | FL002920 | LFD | Treatment |
| LF liv 3 | SA011725 | FL002920 | Liver | Tissue Type |
Collection:
| Collection ID: | CO000269 |
| Collection Summary: | - |
| Collection Protocol Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Sample Type: | Liver, adipose, hypothalamus, muscle, and plasma |
| Collection Method: | Dissection |
| Storage Conditions: | - 80°C |
| Storage Vials: | Eppendorf tubes |
Treatment:
| Treatment ID: | TR000289 |
| Treatment Summary: | Mice were fed 18 weeks on either a low fat diet (LF), high fat diet (HF), or combination of both (SW). |
| Treatment Protocol Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Treatment: | Intervention |
| Treatment Doseduration: | 18 weeks |
| Treatment Vehicle: | animal feed |
| Animal Endp Euthanasia: | 30 weeks |
| Animal Endp Tissue Coll List: | Liver, adipose, hypothalami, muscle, and plasma |
| Animal Endp Tissue Proc Method: | Dissected, placed on dry ice |
Sample Preparation:
| Sampleprep ID: | SP000283 |
| Sampleprep Summary: | Tissues were pulverized on dry ice, enriched with deuterated surrogates, antioxidants (BHT and EDTA), and extracted with MeOH:Water:IPA, transferred to an Ostro plate and the extract was eluted by vacuum. Plasma was added directly to the plate, mixed with the surrogates, antioxidants, and IPA and the extract eluted through. The extracts were dried, reconstituted in 100?L 50:50 methanol:acetonitrile with internal standards 1?phenyl?3?hexanoic acid urea and 1?cyclohexyl?3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm. |
| Sampleprep Protocol Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Extraction Method: | Ostro plate |
| Extract Concentration Dilution: | 80 µL |
| Extract Cleanup: | Ostro plate |
| Extract Storage: | - 20 °C |
| Sample Resuspension: | 100 µL |
| Sample Spiking: | See sample prep protocol file |
| Organ: | Liver, adipose, hypothalamus, muscle, and plasma |
Combined analysis:
| Analysis ID | AN000406 | AN000407 | AN000408 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | |||
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | |||
| MS Type | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap | ABI Sciex API 4000 QTrap |
| Ion Mode | NEGATIVE | POSITIVE | POSITIVE |
| Units | % Area Ratio | nM | nM |
Chromatography:
| Chromatography ID: | CH000287 |
| Chromatography Summary: | Oxylipin analysis |
| Methods Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Instrument Name: | Waters Acquity |
| Column Temperature: | 60 C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 16 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
| Chromatography ID: | CH000288 |
| Chromatography Summary: | Endocannabinoid analysis |
| Methods Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Instrument Name: | Waters Acquity |
| Column Temperature: | 60 C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 11 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
| Chromatography ID: | CH000289 |
| Chromatography Summary: | Ceramide analysis |
| Methods Filename: | NewmanLab-Mediators-Protocol-PT.pdf |
| Instrument Name: | Waters Acquity |
| Column Temperature: | 60 C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 L |
| Solvent A: | 100% water; 5 mM ammonium formate; 0.2% formic acid |
| Solvent B: | 100% methanol; 0.2% formic acid; 5 mM ammonium formate |
| Analytical Time: | 15 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 L |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 L |
| Sample Loop Size: | 17 L |
MS:
| MS ID: | MS000348 |
| Analysis ID: | AN000406 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | See protocol file |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000349 |
| Analysis ID: | AN000407 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | See protocol file |
| Ion Mode: | POSITIVE |
| MS ID: | MS000350 |
| Analysis ID: | AN000408 |
| Instrument Name: | ABI Sciex API 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | See protocol file |
| Ion Mode: | POSITIVE |
