Return to study ST000270 main page
MB Sample ID: SA012129
| Local Sample ID: | Lamba_TB-04-1048 |
| Subject ID: | SU000290 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | 9606 |
| Weight Or Weight Range: | 0-21 |
| Human Exclusion Criteria: | Pediatric Patients with AML (Age<21 years) |
| Animal Animal Supplier: | Aml patients >21 years of age |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000290 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | 9606 |
| Weight Or Weight Range: | 0-21 |
| Human Exclusion Criteria: | Pediatric Patients with AML (Age<21 years) |
| Animal Animal Supplier: | Aml patients >21 years of age |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Lamba_TB-04-1048 | SA012129 | FL003021 | Diagnosis -No treatment | Treatment |
Collection:
| Collection ID: | CO000284 |
| Collection Summary: | Blood samples were collected at diagnosis and after patients achieved remission after chemotherapy |
| Sample Type: | Blood collected and serum separated |
| Collection Location: | Memphis |
| Storage Conditions: | -80 C |
| Blood Serum Or Plasma: | Blood Serum |
Treatment:
| Treatment ID: | TR000304 |
| Treatment Summary: | Most of the samples are at diagnosis without any treatment, 7 samples were received after pateints had treatment with cytarabine, Daunorubicin, and etoposide |
Sample Preparation:
| Sampleprep ID: | SP000298 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
| Analysis ID | AN000432 | AN000433 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000302 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000373 |
| Analysis ID: | AN000432 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000374 |
| Analysis ID: | AN000433 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
