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MB Sample ID: SA013119
Local Sample ID: | scf_03-M3 |
Subject ID: | SU000307 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Animal Animal Supplier: | Jackson Lab |
Animal Housing: | McKnight Brain Institute, UF |
Animal Light Cycle: | 12 hr light/12 hr dark |
Animal Feed: | ad libtum |
Animal Water: | ad libtum |
Animal Inclusion Criteria: | males only |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000307 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Animal Animal Supplier: | Jackson Lab |
Animal Housing: | McKnight Brain Institute, UF |
Animal Light Cycle: | 12 hr light/12 hr dark |
Animal Feed: | ad libtum |
Animal Water: | ad libtum |
Animal Inclusion Criteria: | males only |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
scf_03-M3 | SA013119 | FL003103 | mdx | Strain |
Collection:
Collection ID: | CO000301 |
Collection Summary: | Muscles extracted under isoflurane, snap frozen with liquid nitrogen, stored at -80C |
Collection Protocol Comments: | L3-190 |
Sample Type: | Muscle |
Collection Method: | muscle extraction, frozen |
Collection Location: | UF, musle physiology laboratory |
Collection Frequency: | once/mouse |
Collection Duration: | Total ~5 min |
Collection Time: | no dose |
Volumeoramount Collected: | entire muscles extracted |
Storage Conditions: | -80C freezer |
Collection Vials: | 2 ml vials |
Storage Vials: | 2 ml vials |
Collection Tube Temp: | -80C |
Additives: | none |
Blood Serum Or Plasma: | none |
Tissue Cell Identification: | none |
Tissue Cell Quantity Taken: | none |
Treatment:
Treatment ID: | TR000321 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP000315 |
Sampleprep Summary: | NAD/ATP or NADH |
Sampleprep Protocol Filename: | SB_NAD-ATP_Assay.pdf, SB_NADH_Assay_Agilent.pdf |
Combined analysis:
Analysis ID | AN000456 | AN000457 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ |
Ion Mode | POSITIVE | POSITIVE |
Units | pmol/mg dry powder | pmol/mg dry powder |
Chromatography:
Chromatography ID: | CH000325 |
Chromatography Summary: | NAD-ATP method |
Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000326 |
Chromatography Summary: | NAD-ATP method |
Methods Filename: | SB_NADH_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 30 |
Flow Rate: | 0.54 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 6 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000396 |
Analysis ID: | AN000456 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | NAD/ATP |
Ion Mode: | POSITIVE |
Analysis Protocol File: | SB_NAD-ATP_Assay_Agilent.pdf |
MS ID: | MS000397 |
Analysis ID: | AN000457 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | NADH |
Ion Mode: | POSITIVE |
Analysis Protocol File: | SB_NADH_Assay_Agilent.pdf |