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MB Sample ID: SA014470
| Local Sample ID: | ND7 |
| Subject ID: | SU000336 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000336 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ND7 | SA014470 | FL003281 | ND | Diet |
Collection:
| Collection ID: | CO000330 |
| Collection Summary: | Plasma 200uL were extracted with 1mL of 80% MeOH |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000350 |
| Treatment Summary: | Seven-week-old C57BL6J male mice were purchased from Daehan bio-link (Chungbuk, Republic of Korea). All mice were acclimated for 1 week under controlled conditions (temperature: 25 ± 2°C, relative humidity: 50 ± 5%, and 12 h light/dark cycle). The animals had free access to a normal diet (AIN-76A, Research Diets, Inc., NJ, USA) and water. After 1 week, the mice were randomly distributed into 5 groups: (1) group normal-diet (ND) (n = 11) was fed a normal diet for 6 weeks, (2) group HFD (named HD; n = 10) was fed 60 kcal% fat (D1242, Research Diets, Inc., NJ, USA) for 6 weeks, (3) group HFD with xenical administration (HX, 50 mg?kg?1?day?1) n = 10, 6 weeks, (4) group HFD with CLM administration (HCLM, 2 g?kg?1?day?1) n = 10, 6 weeks; and (5) group HFD with FCLM (CLM fermented for 60 h) administration (HFCLM, 2 g?kg?1?day?1) n = 8, 6 weeks. In this study, we used xenical as a positive control for antiobesity effects. Xenical, CLM, and FCLM dissolved with saline were orally administered everyday into the stomach with an oral zonde needle. And, the equal volume of saline were applied for ND and HD groups. During the experimental periods, the feed intake and the body weight of the mice were measured daily. |
| Treatment Protocol Filename: | DongHo_20160115_025706_PR_CO_The_animal_experiment.docx |
Sample Preparation:
| Sampleprep ID: | SP000344 |
| Sampleprep Summary: | For blood collection, the mice were sacrificed by cardiac puncture. Plasma (100 µL) was subjected to extraction with cold methanol (500 µL) on a MM400 mixer mill (Retsch®, Haan, Germany) with the frequency 30 s?1 for 5 min. The suspension was centrifuged at 12,578 g for 10 min at 4°C. The supernatant was filtered through a 0.2-µm PTEE filter and evaporated in a speed vacuum concentrator. The final concentration of the plasma was 5 mg/mL in methanol for the UPLC-Q-TOF-MS analysis. |
Combined analysis:
| Analysis ID | AN000502 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Micromass QTOF Premier |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000354 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000438 |
| Analysis ID: | AN000502 |
| Instrument Name: | Waters Micromass QTOF Premier |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
