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MB Sample ID: SA014505

Local Sample ID:Seed-133-3A
Subject ID:SU000340
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Species Group:Plant

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU000340
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
Species Group:Plant

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Seed-133-3ASA014505FL003288133Treatment

Collection:

Collection ID:CO000334
Collection Summary:Plants are 30-35d old. They were harvested in liquid N2, grounded to fine powder, distributed to individual falcon tubes and kept at dry ice before handing over the sample to core facility.
Collection Protocol Filename:StudyDesign-AvinashSrivastava-82514.pdf
Sample Type:Seeds

Treatment:

Treatment ID:TR000354
Treatment Summary:Single treatment gene impact on plant metabolites and compared to WT control: 1. Control (A,B,C,D,E,F total 6 replicates) 2. Sample 1 (A,B,C,D,E,F total 6 replicates) 3. Sample 2. (A,B,C,D,E,F total 6 replicates)
Treatment Protocol Filename:StudyDesign-AvinashSrivastava-82514.pdf

Sample Preparation:

Sampleprep ID:SP000348
Sampleprep Summary:-
Sampleprep Protocol Filename:Sample_Prep_Avinash_Srivastava_mx_188894.pdf

Combined analysis:

Analysis ID AN000508 AN000509
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Unspecified Unspecified
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6550 QTOF
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000358
Chromatography Summary:UPLC
Methods Filename:Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf
Instrument Name:Unspecified
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Pressure:450-850 bar
Column Temperature:65 C
Flow Gradient:15% B to 99% B
Flow Rate:0.6 mL/min
Internal Standard:See data dictionary
Retention Time:See data dictionary
Sample Injection:1.67 uL
Solvent A:60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate
Analytical Time:13 min
Capillary Voltage:3500
Time Program:15 min
Weak Wash Solvent Name:Isopropanol
Weak Wash Volume:5 seconds
Strong Wash Solvent Name:Isopropanol
Target Sample Temperature:Autosampler temp 4 C
Randomization Order:Excel
Chromatography Type:Reversed phase

MS:

MS ID:MS000444
Analysis ID:AN000508
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3500
Collision Gas:Nitrogen
Dry Gas Flow:8 l/min
Dry Gas Temp:325
Fragment Voltage:120
Fragmentation Method:Auto MS/MS
Ion Source Temperature:325
Ion Spray Voltage:1000
Ionization:Pos
Precursor Type:Intact Molecule
Reagent Gas:Nitrogen
Source Temperature:325 C
Desolvation Gas Flow:11 l/min
Desolvation Temperature:350 C
Nebulizer:35 psig
Octpole Voltage:750
Resolution Setting:Extended Dynamic Range
Scan Range Moverz:60-1700 Da
Scanning Cycle:2 Hz
Scanning Range:60-1700 Da
Skimmer Voltage:65
  
MS ID:MS000445
Analysis ID:AN000509
Instrument Name:Agilent 6550 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:3500
Collision Gas:Nitrogen
Dry Gas Flow:8 l/min
Dry Gas Temp:325
Fragment Voltage:120
Fragmentation Method:Auto MS/MS
Ion Source Temperature:325
Ion Spray Voltage:1000
Ionization:Pos
Precursor Type:Intact Molecule
Reagent Gas:Nitrogen
Source Temperature:325 C
Desolvation Gas Flow:11 l/min
Desolvation Temperature:350 C
Nebulizer:35 psig
Octpole Voltage:750
Resolution Setting:Extended Dynamic Range
Scan Range Moverz:60-1700 Da
Scanning Cycle:2 Hz
Scanning Range:60-1700 Da
Skimmer Voltage:65
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