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MB Sample ID: SA015187

Local Sample ID:DNA-06
Subject ID:SU000361
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL6
Age Or Age Range:15 weeks
Weight Or Weight Range:20-30g
Gender:Female
Animal Water:with or without Zn
Species Group:Mammal

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Subject:

Subject ID:SU000361
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL6
Age Or Age Range:15 weeks
Weight Or Weight Range:20-30g
Gender:Female
Animal Water:with or without Zn
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
DNA-06SA015187FL003377LiverTissue
DNA-06SA015187FL003377Zn+Treatment

Collection:

Collection ID:CO000355
Collection Summary:DNA will be provided in TE solution. Concentrations are on Metadata sheet.
Collection Protocol Filename:MeDNA_SOP.pdf
Sample Type:Liver DNA in 10mM Tris-HCl, 1 mM EDTA(pH 7.5) & Bone Marrow DNA in 10mM Tris-HCl, 0.5mM EDTA (pH 9.0)
Collection Method:Dissection followed by DNA prep
Collection Location:Our Lab
Collection Frequency:once
Collection Duration:A few minutes.
Volumeoramount Collected:19-20 ul
Storage Conditions:-20C
Storage Vials:eppendorf tubes

Treatment:

Treatment ID:TR000375
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000368
Sampleprep Summary:See file attached.
Sampleprep Protocol Filename:MeDNA_SOP.pdf

Combined analysis:

Analysis ID AN000553
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela
Column Phenomenex Luna C18 (150 x 2mm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units ┬Ámol/l

Chromatography:

Chromatography ID:CH000390
Chromatography Summary:See file
Methods Filename:MeDNA_SOP.pdf
Instrument Name:Thermo Accela
Column Name:Phenomenex Luna C18 (150 x 2mm)
Flow Rate:300 uL/min.
Chromatography Type:Reversed phase

MS:

MS ID:MS000489
Analysis ID:AN000553
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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