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MB Sample ID: SA015204
| Local Sample ID: | Palm32 _11B_T1_015 |
| Subject ID: | SU000362 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000362 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Palm32 _11B_T1_015 | SA015204 | FL003380 | Renal Tissue | Organ |
| Palm32 _11B_T1_015 | SA015204 | FL003380 | PRBC | Treatment |
Collection:
| Collection ID: | CO000356 |
| Collection Summary: | Samples are renal tissues samples. These are from kidneys that were deemed unsuitable for transplantation and discarded. After being discarded these kidneys were brought to our lab where we conducted normothermic machine perfusion on them, where they were perfused with a blood based perfusate to analyze function. The samples are core needle biopsies taken by a surgical instrument. The samples were then snap frozen in liquid nitrogen and stored in -80C till analysis. |
| Collection Protocol Filename: | StudyDesign_IvonnePalma_090815.pdf |
| Sample Type: | Renal Tissue |
Treatment:
| Treatment ID: | TR000376 |
| Treatment Summary: | Human 8A/B, 9A/B, 10A/B, 11A/B were perfused with either a Whole Blood perfusate or a Packed Red Blood Cells. We had previously sent samples from this group but we want to analyze other time points from this group. Group A was perfused with a whole blood perfusate while Group B was perfused with a packed red blood cell perfusate. Human 29 was perfused with a whole blood perfusate and this is from a pediatric patient. We are trying to compare young and old donors and we had previously submitted other pediatric patients. Huma 28A and B were perfused with a packed red blood perfusate. 28A was given Furosemide (a diuretic) to test urine output with acute kidney injury, Human 28B was not given the Furosemide since it is a control. |
| Treatment Protocol Filename: | StudyDesign_IvonnePalma_090815.pdf |
Sample Preparation:
| Sampleprep ID: | SP000369 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Sample_Prep_Ivonne_Palma_mx_234649.pdf |
Combined analysis:
| Analysis ID | AN000554 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6530 |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE |
| Units | Peak height |
Chromatography:
| Chromatography ID: | CH000391 |
| Chromatography Summary: | UPLC |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf |
| Instrument Name: | Agilent 6530 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Pressure: | 450-850 bar |
| Column Temperature: | 65 C |
| Flow Gradient: | 15% B to 99% B |
| Flow Rate: | 0.6 mL/min |
| Sample Injection: | 1.67 uL |
| Solvent A: | 60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate |
| Analytical Time: | 13 min |
| Capillary Voltage: | 3500 |
| Time Program: | 15 min |
| Weak Wash Solvent Name: | Isopropanol |
| Weak Wash Volume: | 5 seconds |
| Strong Wash Solvent Name: | Same |
| Target Sample Temperature: | Autosampler temp 4 C |
| Randomization Order: | Excel |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000490 |
| Analysis ID: | AN000554 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs Lipidomics runs are performed in negative and positve mode (two filenames) |
| Ion Mode: | NEGATIVE |
| Capillary Voltage: | 3500 |
| Collision Gas: | Nitrogen |
| Dry Gas Flow: | 8 l/min |
| Dry Gas Temp: | 325 |
| Fragment Voltage: | 175 |
| Fragmentation Method: | Auto MS/MS |
| Ion Source Temperature: | 200 |
| Ion Spray Voltage: | 1000 |
| Ionization: | Neg |
| Precursor Type: | Intact Molecule |
| Reagent Gas: | Nitrogen |
| Source Temperature: | 325 C |
| Dataformat: | .d |
| Desolvation Gas Flow: | 11 l/min |
| Desolvation Temperature: | 350 C |
| Nebulizer: | 35 psig |
| Octpole Voltage: | 750 |
| Scan Range Moverz: | 60-1700 Da |
| Scanning Cycle: | 2 Hz |
| Scanning Range: | 60-1700 Da |
| Skimmer Voltage: | 65 |
