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MB Sample ID: SA015442

Local Sample ID:UBUF-00122
Subject ID:SU000372
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6N|C57BL/6N Twitcher
Age Or Age Range:P15-P22 days
Species Group:Mammal

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Subject:

Subject ID:SU000372
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6N|C57BL/6N Twitcher
Age Or Age Range:P15-P22 days
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
UBUF-00122SA015442FL003442P22age
UBUF-00122SA015442FL003442Twitcher 8genotype

Collection:

Collection ID:CO000366
Collection Summary:None
Collection Protocol ID:COL_PROT
Collection Protocol Filename:pathogenesis_Krabbe_collection_protocol.pdf
Sample Type:Hindbrain (pons, medulla, brainstem, cerebellum)
Volumeoramount Collected:entire hindbrain

Treatment:

Treatment ID:TR000386
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000379
Sampleprep Summary:None
Processing Method:Homogenization
Processing Storage Conditions:On Ice
Organ:Brain
Organ Specification:Hindbrain

Combined analysis:

Analysis ID AN000572 AN000573 AN000574
Analysis type MS MS MS
Chromatography type Unspecified Unspecified GC
Chromatography system Waters ACQUITY UPLC Waters ACQUITY UPLC See document
Column See doc See doc See document
MS Type ESI ESI EI
MS instrument type Single quadrupole Single quadrupole Single quadrupole
MS instrument name Thermo LTQ Thermo LTQ Thermo Trace DSQ
Ion Mode POSITIVE NEGATIVE POSITIVE
Units Peak height Peak height Peak height

Chromatography:

Chromatography ID:CH000409
Chromatography Summary:Experiments were conducted according to the protocols approved by the Institutional Animal Care and Use Committee of University at Buffalo and Roswell Park Cancer Institute. Twitcher mice were maintained on the background of C57BL/6N. Breeder C57BL/6N mice were purchased from Charles River. Freshly collected hindbrain regions from twitcher and WT littermate mice at P8, P15, P22, and P34 days were transferred into polypropylene cryovials, and then frozen in liquid nitrogen and stored at - 80°C until the analysis.
Methods Filename:pathogenesis_Krabbe_protocol.pdf
Instrument Name:Waters ACQUITY UPLC
Column Name:See doc
Chromatography Type:Unspecified
  
Chromatography ID:CH000410
Chromatography Summary:Experiments were conducted according to the protocols approved by the Institutional Animal Care and Use Committee of University at Buffalo and Roswell Park Cancer Institute. Twitcher mice were maintained on the background of C57BL/6N. Breeder C57BL/6N mice were purchased from Charles River. Freshly collected hindbrain regions from twitcher and WT littermate mice at P8, P15, P22, and P34 days were transferred into polypropylene cryovials, and then frozen in liquid nitrogen and stored at - 80°C until the analysis.
Methods Filename:pathogenesis_Krabbe_protocol.pdf
Instrument Name:See document
Column Name:See document
Solvent A:5% phenyl
Chromatography Type:GC

MS:

MS ID:MS000508
Analysis ID:AN000572
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:pathogenesis_Krabbe_protocol.pdf
  
MS ID:MS000509
Analysis ID:AN000573
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:pathogenesis_Krabbe_protocol.pdf
  
MS ID:MS000510
Analysis ID:AN000574
Instrument Name:Thermo Trace DSQ
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Analysis Protocol File:pathogenesis_Krabbe_protocol.pdf
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