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MB Sample ID: SA016514
Local Sample ID: | 32_UK49 |
Subject ID: | SU000388 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | tissue_slice |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000388 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Subject Comments: | tissue_slice |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
32_UK49 | SA016514 | FL003716 | 13C6Glc | Treatment |
32_UK49 | SA016514 | FL003716 | nontumor | Tissue type |
Collection:
Collection ID: | CO000382 |
Collection Summary: | 2 methods were used: Precellys homogenization (Precellys_homogenization_SOP-2015-04-09.pdf, measurements: IC-FTMS1) and Retsch homogenization (protocol: Retsch_homogenization_SOP-2015-09-27.pdf, measurements: IC-FTMS2) |
Collection Protocol Filename: | Precellys_homogenization_SOP-2015-04-09.pdf Retsch_homogenization_SOP-2015-09-27.pdf |
Collection Protocol Comments: | Precellys_homogenization_SOP-2015-04-09.pdf (IC-FTMS1), Retsch_homogenization_SOP-2015-09-27.pdf (IC-FTMS2) |
Sample Type: | Lung |
Treatment:
Treatment ID: | TR000402 |
Treatment Summary: | none |
Treatment Protocol Filename: | SIRM_ex_vivo_tissue_slices_protocol__Bio-protocol_6_e1730__2016.pdf |
Treatment Protocol Comments: | ex vivo tissue slice experiment with isotopic labeling. |
Sample Preparation:
Sampleprep ID: | SP000395 |
Sampleprep Summary: | Preparation of polar samples for GCMS, NMR, and IC-FTMS; Protein extraction and quantification |
Sampleprep Protocol Filename: | Extract_Polar_Lipid_Prot_Fan.pdf Extract_Protein_Quant_Fan.pdf |
Combined analysis:
Analysis ID | AN000600 | AN000601 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Ion Chromatography | Ion Chromatography |
Chromatography system | Thermo Dionex ICS-5000+ | Thermo Dionex ICS-5000+ |
Column | Unspecified | Unspecified |
MS Type | ESI | ESI |
MS instrument type | FT-ICR-MS | FT-ICR-MS |
MS instrument name | Thermo Fusion Tribrid Orbitrap | Thermo Fusion Tribrid Orbitrap |
Ion Mode | NEGATIVE | NEGATIVE |
Units | normalized corrected peak area | normalized corrected peak area |
Chromatography:
Chromatography ID: | CH000427 |
Instrument Name: | Thermo Dionex ICS-5000+ |
Column Name: | Unspecified |
Chromatography Type: | Ion Chromatography |
MS:
MS ID: | MS000533 |
Analysis ID: | AN000600 |
Instrument Name: | Thermo Fusion Tribrid Orbitrap |
Instrument Type: | FT-ICR-MS |
MS Type: | ESI |
MS Comments: | IC-FTMS1: Data were adjusted as follows: 1)Get raw XIC peak areas from TF33 -> 2)correct for protein content by adjusting the signals according by wet weight -> 3) correct for NA abundance. Some compounds were assigned using a sperate standard run, others were assigned because they were the only or the highest peak in the extracted ion chromatogram. (XIC). |
Ion Mode: | NEGATIVE |
MS ID: | MS000534 |
Analysis ID: | AN000601 |
Instrument Name: | Thermo Fusion Tribrid Orbitrap |
Instrument Type: | FT-ICR-MS |
MS Type: | ESI |
MS Comments: | IC-FTMS2: Data were adjusted as follows: 1)Get raw XIC peak areas from TF33 -> 2)correct for protein content by reconstituting the volumes according to tissue wet weight -> 3) correct for NA abundance. Some compounds were assigned using a sperate standard run, others were assigned because they were the only or the highest peak in the extracted ion chromatogram. (XIC). |
Ion Mode: | NEGATIVE |