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MB Sample ID: SA019519
| Local Sample ID: | Vixen-A |
| Subject ID: | SU000427 |
| Subject Type: | Animal |
| Subject Species: | Felis catus |
| Taxonomy ID: | 9685 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000427 |
| Subject Type: | Animal |
| Subject Species: | Felis catus |
| Taxonomy ID: | 9685 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Vixen-A | SA019519 | FL004744 | Asthamtic | Type |
Collection:
| Collection ID: | CO000421 |
| Collection Summary: | 1D 1H NMR spectra were collected using W5-WATERGATE water suppression supplemented by presaturation of the water resonance at minimal power. |
| Collection Protocol Filename: | protocol.pdf |
| Sample Type: | EBC (Exhaled breath condensate) and BALF (bronchoalveolar lavage fluid) |
Treatment:
| Treatment ID: | TR000441 |
| Treatment Summary: | The care of the 53 cats in the study followed the NIH Guide for the Care and Use of Laboratory Animals. The University of Missouri Animal Care and Use Committee approved the study design (ACUC protocols #6912 and 7891). In order to induce allergic asthma, on day 0, cats <1 year of age were administered 12 µg of Bermuda grass allergen (BGA) in 10 mg of alum and 100 ng Bordetella pertussis toxin (both subcutaneously). On day 14, intranasal BGA (75 µg of BGA in 200 µL of phosphate-buffered saline) was given; and on day 21, 12 µg of BGA in 10 mg alum was injected subcutaneously. On day 28, the formation of wheals on an intradermal test confirmed sensitization to BGA. For the next 2 weeks, intensive aerosol challenges were administered to all cats with BGA (500 µg of BGA in 4 mL of phosphate-buffered saline) over 10 min in awake and unrestrained cats in a sealed plastic chamber. A nebulizer (Acorn nebulizer, model 646, Devilbis Health Care, Somerset, PA) delivered an aerosol of the BGA solution at an air flow rate of 9.3 l /min. An air compressor (Easy Air 15, Precision Medical, Inc., Northampton, PA) supplied the compressed air flow at a pressure of 2.93 kg/cm2. At week 6, samples were collected 24 h after challenge with BGA aerosol (see below). |
| Treatment Compound: | Bermuda grass allergen (BGA) |
Sample Preparation:
| Sampleprep ID: | SP000434 |
| Sampleprep Summary: | In all cats, Exhaled breath condensate (EBC)and Bronchoalveolar lavage fluid (BALF) and were collected before (day 0) and after (week 6) sensitization to BGA. The EBC was collected non-invasively just prior to BALF collection by placing cats in a 25 L plexiglass chamber for 20-30 min according to a previously published design with modifications [29]. BALF was collected in a blind fashion under anesthesia using an 8 French red rubber catheter passed through an endotracheal tube according to a previously described protocol [30]. The percentage of eosinophils in each BALF modified Wright’s stained cytospin was determined by counting 200 nucleated cells, with an asthmatic phenotype defined as >17% eosinophils.The EBC and the supernatant of centrifuged BALF remaining were promptly stored at -80 °C until further analysis. NMR spectra of the EBC were measured for use in training and evaluating statistical approaches and biomarkers. |
Analysis:
| MB Sample ID: | SA019519 |
| Analysis ID: | AN000646 |
| Laboratory Name: | Van Doren Lab |
| Analysis Type: | NMR |
| Software Version: | Topspin 3.1 |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000068 |
| Analysis ID: | AN000646 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 800 MHz |
| Binned Increment: | 0.02 |
| Binned Data Excluded Range: | 4.5-5.38 |
