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MB Sample ID: SA024334
| Local Sample ID: | tx4 |
| Subject ID: | SU000495 |
| Subject Type: | Human leukemia cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | HL-60 |
| Subject Comments: | immortalized |
| Cell Counts: | 10 million per sample |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000495 |
| Subject Type: | Human leukemia cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | HL-60 |
| Subject Comments: | immortalized |
| Cell Counts: | 10 million per sample |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| tx4 | SA024334 | FL005885 | MDH inhib | Treatment |
Collection:
| Collection ID: | CO000489 |
| Collection Summary: | Cells were centrifuged to remove growth media/drug and either flash frozen or washed 1x in PBS. The cells were washed for the targeted assay but not for the global assay. |
| Sample Type: | cell |
| Storage Conditions: | flash frozen |
| Tissue Cell Quantity Taken: | 10 million per sample |
Treatment:
| Treatment ID: | TR000509 |
| Treatment Summary: | Cells were treated for 15hr with 1 micromolar of drug or vehicle |
| Cell Growth Container: | flasks |
| Cell Growth Rate: | ~24hr per division |
| Cell Media: | RPMI with 10% FBS |
| Cell Envir Cond: | 5% CO2 incubator |
| Cell Media Lastchanged: | 15 hr |
Sample Preparation:
| Sampleprep ID: | SP000502 |
| Sampleprep Summary: | Amino Acid|Global |
| Sampleprep Protocol Filename: | SB_OA_Assay.pdf Appendix_C_Protein_DNA_quant.pdf |
| Sample Spiking: | Appendix A - Internal Standard Prep GLCMS.pdf |
Combined analysis:
| Analysis ID | AN000738 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE |
| Units | Total nmoles (with relative protein adjustment) |
Chromatography:
| Chromatography ID: | CH000531 |
| Chromatography Summary: | Amino acids |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000655 |
| Analysis ID: | AN000738 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
