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MB Sample ID: SA025775
| Local Sample ID: | RIC-22 |
| Subject ID: | SU000518 |
| Subject Type: | LC-MS & GC-MS |
| Subject Species: | Nicotiana tabacum |
| Taxonomy ID: | 4097 |
| Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000518 |
| Subject Type: | LC-MS & GC-MS |
| Subject Species: | Nicotiana tabacum |
| Taxonomy ID: | 4097 |
| Species Group: | Plant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| RIC-22 | SA025775 | FL006595 | control | Treatment |
| RIC-22 | SA025775 | FL006595 | - | Sample_description |
Collection:
| Collection ID: | CO000512 |
| Collection Summary: | LC-MS & GC-MS |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR000532 |
| Treatment Summary: | Nicotiana tabacum (White Burley) plants were inoculated by M-CMV infected plants. |
Sample Preparation:
| Sampleprep ID: | SP000525 |
| Sampleprep Summary: | Samples were prepared using the automated MicroLab STAR® system (Hamilton Company, Salt Lake City, UT). A 6-mg quantity of each lyophilized leaf sample was thawed on ice and extracted using the automated MicroLab STAR system in 400 L of methanol (LC-MS Ultra CHROMASOLV®, Riedel-de Haёn) containing recovery standards. The samples were prepared using a proprietary series of organic and aqueous extractions to remove the protein fraction while maximizing the recovery of small molecules. The resulting extract was divided into two fractions; one for analysis by liquid chromatography (LC) and one for analysis by gas chromatography (GC). The samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. Each sample was then frozen and dried under vacuum, followed by preparation for either LC/MS or GC/MS. |
Combined analysis:
| Analysis ID | AN000763 | AN000764 | AN000765 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | GC |
| Chromatography system | Waters Acquity | Waters Acquity | hermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer |
| Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | 5% phenyldimethyl silicone column |
| MS Type | ESI | ESI | EI |
| MS instrument type | Single quadrupole | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo LTQ | Thermo LTQ | Thermo Trace DSQ |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | Normalized scale | Normalized scale | Normalized scale |
Chromatography:
| Chromatography ID: | CH000549 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000550 |
| Instrument Name: | hermo-Finnigan Trace DSQ fast-scanning single-quadrupole mass spectrometer |
| Column Name: | 5% phenyldimethyl silicone column |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000675 |
| Analysis ID: | AN000763 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000676 |
| Analysis ID: | AN000764 |
| Instrument Name: | Thermo LTQ |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000677 |
| Analysis ID: | AN000765 |
| Instrument Name: | Thermo Trace DSQ |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
