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MB Sample ID: SA025799
| Local Sample ID: | LongDNP_002 |
| Subject ID: | SU000519 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Subject Comments: | Long Evans Rat |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000519 |
| Subject Type: | Animal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Subject Comments: | Long Evans Rat |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| LongDNP_002 | SA025799 | FL006024 | acute | drug admin |
Collection:
| Collection ID: | CO000513 |
| Collection Summary: | harvested, rinsed with cold saline, left hemisphere flash frozen in liquid N2 |
| Sample Type: | Tissue |
Treatment:
| Treatment ID: | TR000533 |
| Treatment Summary: | Acute injection = 10mg/kg/cc cocaine i.p, Acute Control = no injection, Withdrawal = 10mg/kg/cc cocaine i.p daily for 10 days_3days withdrawal, Withdrawal control = 1ml/kg saline i.p daily for 10 days_3 days withdrawal |
Sample Preparation:
| Sampleprep ID: | SP000526 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | SB_OA_Assay.pdf |
Combined analysis:
| Analysis ID | AN000766 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantiva QQQ |
| Ion Mode | POSITIVE |
| Units | nmol/mg dry powder |
Chromatography:
| Chromatography ID: | CH000551 |
| Methods Filename: | SB_OA_Assay.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000678 |
| Analysis ID: | AN000766 |
| Instrument Name: | Thermo Quantiva QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
