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MB Sample ID: SA028743
| Local Sample ID: | kim_ESC-H |
| Subject ID: | SU000576 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | B6/CBA |
| Cell Primary Immortalized: | B6/CBA |
| Cell Passage Number: | Cultured primary cells |
| Cell Counts: | 16-18 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000576 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | B6/CBA |
| Cell Primary Immortalized: | B6/CBA |
| Cell Passage Number: | Cultured primary cells |
| Cell Counts: | 16-18 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| kim_ESC-H | SA028743 | FL007014 | Untreated | Treatment |
Collection:
| Collection ID: | CO000570 |
| Collection Summary: | none |
| Collection Protocol Comments: | Cells were scraped off on ice in pbs |
| Sample Type: | Cultured primary cells |
| Collection Location: | Cold Room |
| Collection Frequency: | two plates at a time |
| Collection Duration: | 15 mins |
| Volumeoramount Collected: | In 5ml cold PBS |
| Storage Conditions: | N2 snap frozen, stored at -80 |
| Collection Vials: | Falcon tube and eppendorf tube |
| Storage Vials: | Eppendorf tube |
| Collection Tube Temp: | <4C |
| Additives: | None |
| Tissue Cell Quantity Taken: | 5-10mill |
Treatment:
| Treatment ID: | TR000590 |
| Treatment Summary: | No treatment |
| Plant Humidity: | - |
Sample Preparation:
| Sampleprep ID: | SP000583 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_Global_LCMS.pdf |
Combined analysis:
| Analysis ID | AN000849 | AN000850 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | None | None |
| Column | None | None |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH000606 |
| Methods Filename: | Metabolomics_Global_LCMS.pdf |
| Instrument Name: | None |
| Column Name: | None |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000750 |
| Analysis ID: | AN000849 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000751 |
| Analysis ID: | AN000850 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | HESI |
| Ion Mode: | NEGATIVE |
