Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA031831

Local Sample ID:	HF04
Subject ID:	SU000610
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Subject:

Subject ID:	SU000610
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
HF04	SA031831	FL007197	heart failure	factor

Collection:

Collection ID:	CO000604
Collection Summary:	The samples were collected in Dr. Bruce Johnson lab, at the Department of Cardiovascular Diseases, Mayo Clinic. EBC samples were collected using Jaeger EcoScreen cooling unit (-20°C). Patients were breathing through mouth piece while wearing a nose clip during 30 minutes. Saliva was collected from the same patients during last 15 minutes. The samples were frozen immediately after collection.
Sample Type:	Breath

Treatment:

Treatment ID:	TR000624
Treatment Summary:	We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points.

Treatment ID:

TR000624

Treatment Summary:

We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points.

Sample Preparation:

Sampleprep ID:	SP000617
Sampleprep Summary:	EBC: Transfer 800μl aliquots of EBC samples into a 1.5ml microcentrifuge tubes and dry down (lyophilize) each sample in a centrifugal vacuum evaporator for 4-6 h. Apply no heating during the drying process. In the dried EBC samples add 250μl of phosphate buffer and 250μl of 4mM TSP-d4 solution. Vortex for 20 s. Transfer the solution to 5mm NMR tube.

Analysis:

MB Sample ID:	SA031831
Analysis ID:	AN000902
Analysis Type:	NMR
Num Factors:	2
Num Metabolites:	9
Units:	uM

NMR:

NMR ID:	NM000100
Analysis ID:	AN000902
Instrument Name:	Bruker 600 MHz Avance III HD spectrometer
Instrument Type:	FT-NMR
NMR Experiment Type:	1D 1H
Spectrometer Frequency:	600 MHz