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MB Sample ID: SA033515
| Local Sample ID: | CH10WB |
| Subject ID: | SU000630 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 |
| Age Or Age Range: | 10 weeks and 12-24 months (young & old) |
| Weight Or Weight Range: | ~25 g and ~34 g |
| Gender: | Male |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000630 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl6 |
| Age Or Age Range: | 10 weeks and 12-24 months (young & old) |
| Weight Or Weight Range: | ~25 g and ~34 g |
| Gender: | Male |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CH10WB | SA033515 | FL007450 | DVT old | Condition |
Collection:
| Collection ID: | CO000624 |
| Collection Summary: | None |
| Collection Protocol Filename: | SOP_for_whole_blood_collection_exp._animals_v1.3.pdf |
| Sample Type: | Whole blood |
| Collection Tube Temp: | 4C |
| Additives: | Sodium heparin |
| Blood Serum Or Plasma: | Whole blood |
Treatment:
| Treatment ID: | TR000644 |
| Treatment Summary: | None |
| Animal Vet Treatments: | Electrolytic inferior vena cava (IVC) model (EIM to induce thrombogenesis |
| Animal Anesthesia: | 2-5% isoflurane with 100% oxygen |
| Animal Fasting: | No |
| Animal Endp Euthanasia: | Per IUCUCA and 2d following induction of VT |
| Animal Endp Tissue Coll List: | Blood sampling, thrombus and vein wall |
Sample Preparation:
| Sampleprep ID: | SP000637 |
| Sampleprep Summary: | Methanol:cholorform extraction |
| Sampleprep Protocol Filename: | Wakefield_Mouse_WB_Extraction_Protocol.pdf |
| Sampleprep Protocol Comments: | Lipid fractions saved, stored at -20°C |
| Processing Method: | Methanol:Chloroform Extraction |
| Processing Storage Conditions: | 0-4°C |
| Extraction Method: | Methanol:chloroform extraction |
| Extract Cleanup: | N/A |
| Extract Storage: | -80°C |
Analysis:
| MB Sample ID: | SA033515 |
| Analysis ID: | AN000928 |
| Laboratory Name: | University of Michigan Biochemical NMR Core Laboratory |
| Analysis Type: | NMR |
| Software Version: | VNMRJ 3.2 |
| Operator Name: | Michael Finkel |
| Data Format: | .fid |
| Num Factors: | 4 |
| Num Metabolites: | 54 |
| Units: | uM |
NMR:
| NMR ID: | NM000104 |
| Analysis ID: | AN000928 |
| Instrument Name: | Agilent 500/54 Premium Shielded VNMRS system |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Standard Concentration: | 10% (~0.5mM) |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | ONE-Probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming) |
| Pulse Sequence: | 1D-NOESY |
| Water Suppression: | saturation at 80 Hz induced field strength |
| Pulse Width: | 5.5ms |
| Offset Frequency: | around -178Hz |
| Chemical Shift Ref Cpd: | 6mM Formate |
| Temperature: | 25°C |
| Number Of Scans: | 32 |
