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MB Sample ID: SA033522
Local Sample ID: | CH25WB |
Subject ID: | SU000630 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl6 |
Age Or Age Range: | 10 weeks and 12-24 months (young & old) |
Weight Or Weight Range: | ~25 g and ~34 g |
Gender: | Male |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000630 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl6 |
Age Or Age Range: | 10 weeks and 12-24 months (young & old) |
Weight Or Weight Range: | ~25 g and ~34 g |
Gender: | Male |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CH25WB | SA033522 | FL007451 | DVT young | Condition |
Collection:
Collection ID: | CO000624 |
Collection Summary: | None |
Collection Protocol Filename: | SOP_for_whole_blood_collection_exp._animals_v1.3.pdf |
Sample Type: | Whole blood |
Collection Tube Temp: | 4C |
Additives: | Sodium heparin |
Blood Serum Or Plasma: | Whole blood |
Treatment:
Treatment ID: | TR000644 |
Treatment Summary: | None |
Animal Vet Treatments: | Electrolytic inferior vena cava (IVC) model (EIM to induce thrombogenesis |
Animal Anesthesia: | 2-5% isoflurane with 100% oxygen |
Animal Fasting: | No |
Animal Endp Euthanasia: | Per IUCUCA and 2d following induction of VT |
Animal Endp Tissue Coll List: | Blood sampling, thrombus and vein wall |
Sample Preparation:
Sampleprep ID: | SP000637 |
Sampleprep Summary: | Methanol:cholorform extraction |
Sampleprep Protocol Filename: | Wakefield_Mouse_WB_Extraction_Protocol.pdf |
Sampleprep Protocol Comments: | Lipid fractions saved, stored at -20°C |
Processing Method: | Methanol:Chloroform Extraction |
Processing Storage Conditions: | 0-4°C |
Extraction Method: | Methanol:chloroform extraction |
Extract Cleanup: | N/A |
Extract Storage: | -80°C |
Analysis:
MB Sample ID: | SA033522 |
Analysis ID: | AN000928 |
Laboratory Name: | University of Michigan Biochemical NMR Core Laboratory |
Analysis Type: | NMR |
Software Version: | VNMRJ 3.2 |
Operator Name: | Michael Finkel |
Data Format: | .fid |
Num Factors: | 4 |
Num Metabolites: | 54 |
Units: | uM |
NMR:
NMR ID: | NM000104 |
Analysis ID: | AN000928 |
Instrument Name: | Agilent 500/54 Premium Shielded VNMRS system |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Standard Concentration: | 10% (~0.5mM) |
Spectrometer Frequency: | 500 MHz |
NMR Probe: | ONE-Probe |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Auto shim (gradient shimming) |
Pulse Sequence: | 1D-NOESY |
Water Suppression: | saturation at 80 Hz induced field strength |
Pulse Width: | 5.5ms |
Offset Frequency: | around -178Hz |
Chemical Shift Ref Cpd: | 6mM Formate |
Temperature: | 25°C |
Number Of Scans: | 32 |