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MB Sample ID: SA035682
| Local Sample ID: | ms5956-13 |
| Subject ID: | SU000658 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000658 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ms5956-13 | SA035682 | FL007536 | H | Sodium level |
| ms5956-13 | SA035682 | FL007536 | B | grouping |
Collection:
| Collection ID: | CO000652 |
| Collection Summary: | The urine samples we have were 24-hour collections occurring in the fourth week on either the low- or the high-sodium intake level. In total, 120 human samples (60 subjects, a low-sodium sample and a high-sodium sample for each subject) will be analyzed. |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR000672 |
| Treatment Summary: | We will analyze urine specimens collected during the Dietary Approaches to Stop Hypertension – Sodium Study (DASH-Sodium or DASH2) clinical trial. More than 400 participants in the study were divided into two groups. One followed a common Western diet, while the other group followed the “healthier” DASH diet. Then each group had three 30 day periods, in random orders, that corresponded to high, intermediate, or low sodium intakes while eating their designated diet. The participants had all of their meals prepared and provided for them during the entire study. It was found that reducing sodium intake significantly reduced blood pressure especially when on the common Western diet. We have obtained urine samples and phenotypic data for a subset of the participants of the DASH2 trial form the NHLBI biospecimen and data repository BioLINCC. We selected two sub-groups of participants on the Western diet. Group A (n=31) exhibited large (>12 mmHg) differences in systolic or diastolic pressure between low- and high-sodium intakes (“salt-sensitive” group). Group B (n=29) showed similar blood pressure (differences <5 mmHg for both systolic and diastolic pressures) between low- and high-sodium intakes (“saltinsensitive” group). |
Sample Preparation:
| Sampleprep ID: | SP000665 |
| Sampleprep Summary: | 2 drops of 6 N HCl was added in every 8 ml urine samples when the 24 hour urine was collected. All urine samples were stored in -80℃ freezer. |
Combined analysis:
| Analysis ID | AN000967 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000692 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000862 |
| Analysis ID: | AN000967 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
