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MB Sample ID: SA042869
| Local Sample ID: | CMC2002-24 |
| Subject ID: | SU000808 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 39-93 years |
| Weight Or Weight Range: | 55-130 KG |
| Gender: | males and females |
| Human Race: | Caucasian (C) and African American (AA) |
| Human Inclusion Criteria: | Suspicion of or confirmed infection; two or more systemic infmammatory response syndrome criteria; hypotension requiring vasopressors despite volume resuscitation; a cumulative vasopressor index of 3 or more after 4 hours of administration; and a SOFA score of at least 5. |
| Human Exclusion Criteria: | Age less than 18; greater than 16h after septic shock recognition; a Do Not Resuscitate order; or known inborn error of metaboloism. |
| Species Group: | Human |
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Subject:
| Subject ID: | SU000808 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 39-93 years |
| Weight Or Weight Range: | 55-130 KG |
| Gender: | males and females |
| Human Race: | Caucasian (C) and African American (AA) |
| Human Inclusion Criteria: | Suspicion of or confirmed infection; two or more systemic infmammatory response syndrome criteria; hypotension requiring vasopressors despite volume resuscitation; a cumulative vasopressor index of 3 or more after 4 hours of administration; and a SOFA score of at least 5. |
| Human Exclusion Criteria: | Age less than 18; greater than 16h after septic shock recognition; a Do Not Resuscitate order; or known inborn error of metaboloism. |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CMC2002-24 | SA042869 | FL008920 | Placebo | Treatment |
Collection:
| Collection ID: | CO000802 |
| Collection Summary: | Serum collected via existing intravenous or arterial catheter |
| Collection Protocol Comments: | T0: collected before carnitine or placebo administration, T24: collected 24h after administration of L-carnitine or placebo (±4h), T48: collected 48h after administration of L-carnitine or placebo (±4h) |
| Sample Type: | Blood serum |
| Collection Method: | via existing intravenous or arterial catheter |
| Collection Frequency: | 1/timepoint, 3 collections/48h |
| Collection Time: | T0: before administration, T24: 24h after administration (±4h), T48: 48h after administration (±4h) |
| Volumeoramount Collected: | 10mL |
| Storage Conditions: | Initial RT then -80°C following processing (within 2h of collection) |
| Collection Vials: | SST: Becton-Dickinson 10mL vacutainer Serum Separator tubes |
| Storage Vials: | Sterile 1mL cryovials |
| Collection Tube Temp: | RT |
| Additives: | None |
| Blood Serum Or Plasma: | Serum |
Treatment:
| Treatment ID: | TR000822 |
| Treatment Summary: | Intravenous carnitine |
| Treatment Protocol Comments: | Non-treatment group: equivalent volume of saline placebo |
| Treatment Compound: | L-carnitine |
| Treatment Route: | 4g bolus followed by an 8g infusion over 12h |
| Treatment Dose: | 12g |
| Treatment Doseduration: | 12h (following initial 4g bolus) |
Sample Preparation:
| Sampleprep ID: | SP000815 |
| Sampleprep Summary: | Methanol:cholorform extraction, filtration |
| Sampleprep Protocol Comments: | Lipid fractions saved, stored at -20°C |
| Processing Method: | Methanol:Chloroform Extraction, filtration |
| Processing Storage Conditions: | 0-4°C |
| Extraction Method: | Methanol:chloroform extraction |
| Extract Enrichment: | Ultra-filtration (3kDa filters) to remove residual protein |
| Extract Cleanup: | N/A |
| Extract Storage: | -80 C |
| Sample Resuspension: | =500 uL Deuterium Oxide (D2O) |
| Sample Spiking: | CaFormate 12mM |
Analysis:
| MB Sample ID: | SA042869 |
| Analysis ID: | AN001244 |
| Laboratory Name: | University of Michigan Biochemical NMR Core Laboratory |
| Analysis Type: | NMR |
| Software Version: | VNMRJ 3.2 |
| Operator Name: | Michael Finkel, Julie Trexel |
| Detector Type: | NMR |
| Data Format: | .FID |
| Num Factors: | 2 |
| Num Metabolites: | 38 |
| Units: | µM |
NMR:
| NMR ID: | NM000105 |
| Analysis ID: | AN001244 |
| Instrument Name: | Agilent 500/54 Premium Shielded VNMRS system |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| NMR Comments: | Experiment dates: 11/7/2013, 11/11/2013, 11/19/2013 |
| Standard Concentration: | 10% (~0.5mM) |
| Spectrometer Frequency: | 500 MHz |
| NMR Probe: | ONE-Probe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 5mm |
| Shimming Method: | Auto shim (gradient shimming) |
| Pulse Sequence: | 1D-NOESY |
| Water Suppression: | saturation at 80 Hz induced field strength |
| Pulse Width: | 5.5ms |
| Power Level: | around -178Hz |
| Chemical Shift Ref Cpd: | Formate |
| Temperature: | 25°C |
| Number Of Scans: | 32 |
| Baseline Correction Method: | manual |
| Chemical Shift Ref Std: | Calcium Formate |
