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MB Sample ID: SA049441
| Local Sample ID: | C-JVGS1-S-01 |
| Subject ID: | SU000904 |
| Subject Type: | Human Chord Blood Serum |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000904 |
| Subject Type: | Human Chord Blood Serum |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| C-JVGS1-S-01 | SA049441 | FL009606 | Pool 1 | Pool Group |
| C-JVGS1-S-01 | SA049441 | FL009606 | Study Sample | Sample Type |
Collection:
| Collection ID: | CO000898 |
| Collection Summary: | Human Chord Blood serum |
| Sample Type: | Serum |
| Storage Conditions: | -80 C |
Treatment:
| Treatment ID: | TR000918 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP000911 |
| Sampleprep Summary: | A total of 200 serum samples and 2 CHEAR Serum Reference Material aliquots were thawed on ice for sample preparation, 150 uL of the thawed serum sample were transferred to labeled tubes on ice where they were mixed with 100 uL of NMR 0.9% Saline Solution containing 2.5 mM formate. Samples were randomly assigned to one of two pools, either Pool 1 or Pool 2. An aliquot of 15 uL of each study sample was added to create their corresponding pools. A 150 uL aliquot was taken from each pool to make 10 aliqouts. Another nine pools from the CHEAR reference material samples were aliquoted and processed along with the study samples and study pools. Pool aliquots were processed identically to the study samples, as described above. Sample tubes were vortexed for 4 minutes on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 mininutes. A volume of 250 uL was taken from each sample supernatant and transferred into a pre-labeled 3mm NMR tube for data acquisition on a 600 MHz spectrometer. |
Analysis:
| MB Sample ID: | SA049441 |
| Analysis ID: | AN001414 |
| Laboratory Name: | David H Murdock Research Institute |
| Analysis Type: | NMR |
| Software Version: | TopSpin 3.2 |
| Operator Name: | Kevin Knagge |
| Detector Type: | NMR |
| Data Format: | fid, 1r |
| Num Factors: | 5 |
| Num Metabolites: | 51 |
| Units: | uM |
NMR:
| NMR ID: | NM000108 |
| Analysis ID: | AN001414 |
| Instrument Name: | Bruker 600 MHz |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| NMR Comments: | Data for this study were acquired on a 600 MHz Bruker NMR spectrometer. Data for the procficiency testing were acquired on a 700 MHz Bruker NMR spectrometer. |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | 1 mM |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5 mm ATMA Cryoprobe |
| NMR Solvent: | D2O |
| NMR Tube Size: | 3 mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | cpmgpr1d |
| Water Suppression: | yes |
| Pulse Width: | 8.99 usec |
| Power Level: | 25.119 W |
| Receiver Gain: | 4 |
| Offset Frequency: | 2817.40 Hz |
| Chemical Shift Ref Cpd: | Formate |
| Temperature: | 298.1 K |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 4.5 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 12.0 ppm |
| Num Data Points Acquired: | 65536 |
| Real Data Points: | 32768 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | yes |
| Apodization: | Lorentzian |
| Baseline Correction Method: | Polynomial |
| Chemical Shift Ref Std: | Formate |
