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MB Sample ID: SA056217
| Local Sample ID: | ECAOMIC.248_T0 |
| Subject ID: | SU000984 |
| Subject Type: | Mammal |
| Subject Species: | Equus caballus |
| Taxonomy ID: | 9796 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000984 |
| Subject Type: | Mammal |
| Subject Species: | Equus caballus |
| Taxonomy ID: | 9796 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ECAOMIC.248_T0 | SA056217 | FL010241 | T0 | Time |
Collection:
| Collection ID: | CO000978 |
| Collection Summary: | Blood samples for metabolome pofiling were obtained from the jugular vein at rest (Basal, T0) and/or immediately after the end of the competition (T1). Pretreatment of the blood samples was carried out immediately after the collection because the access to refrigeration and electrical power supply was available under the field conditions. Briefly, whole blood samples from each horse were collected in sodium fluoride and oxalate tubes for metabolome profiling in order to inhibit further glycolysis that may increase the lactate levels after sampling. Whole blood draw for plasma generation was put at once at 4ºC to minimize the metabolic activity of cells and enzymes and kept the metabolite pattern almost stable. Clotting time at 4ºC was strictly controlled for all samples to avoid cell lyses and affect the components of the metabolome. After clotting at 4ºC, the plasma was separated from the blood cells, subsequently transported to the lab at 4ºC and frozen at -80 °C (no more than 5 h later, in all cases). |
| Sample Type: | Blood (whole) |
Treatment:
| Treatment ID: | TR000998 |
| Treatment Summary: | Two time course comparison of metabolome: T1 (post-endurance competition) relative to T0 (baseline, pre- endurance competition). |
Sample Preparation:
| Sampleprep ID: | SP000991 |
| Sampleprep Summary: | The plasmas were thawed at room temperature. In the 5 mm NMR tubes, 600 µL of plasma was added with 100 µL deuterium oxide for field locking. |
| Sampleprep Protocol Filename: | MO-Blood-Horse.docx |
Analysis:
| MB Sample ID: | SA056217 |
| Analysis ID: | AN001551 |
| Analysis Type: | NMR |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000121 |
| Analysis ID: | AN001551 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 500 MHz |
