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MB Sample ID: SA057269

Local Sample ID:HT29-TX05-S-2
Subject ID:SU000995
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:HT29 cell line (colorectal cancer)

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Subject:

Subject ID:SU000995
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:HT29 cell line (colorectal cancer)

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
HT29-TX05-S-2SA057269FL010317Condition-2Treatment

Collection:

Collection ID:CO000989
Collection Summary:HT29 cells were treated with Sg TX20005 stationary phase, Sg TX20005 exponential phase (negative control), and Sg TX20008 stationary phase (negative control), and media only (baseline control). The cells were washed and aliquoted. One aliquot was pelleted and stored at -80C for metabolomics analysis. DNA was also extracted from the other aliquot for DNA methylation studies.
Sample Type:Cultured cells

Treatment:

Treatment ID:TR001009
Treatment Summary:HT29 cells were co-cultured with S. streptococcus gallolyticus strains TX20008 (stationary phase), TX20005 (Stationary phase) and TX20005 (Exponential phase). Cell storage: After collection ells were stored at -80 C Cell growth container:10 cm plates Cell percent confluence:80% Cell media: F12/DMEM 1:1 + 10% FBS Cell harvesting: Trypisinization

Sample Preparation:

Sampleprep ID:SP001002
Sampleprep Summary:No details provided. HT29 cells
Sampleprep Protocol Filename:GMetabolomics_LCMS_Protocol_092117.pdf
Appendix_A_Internal_Standard_Prep_GLCMS.pdf

Combined analysis:

Analysis ID AN001568 AN001569
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak height peak height

Chromatography:

Chromatography ID:CH001099
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:350ul/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001446
Analysis ID:AN001568
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS001447
Analysis ID:AN001569
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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