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MB Sample ID: SA059448
| Local Sample ID: | RB14 B05 |
| Subject ID: | SU001016 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
| Human Race: | Hispanic and Caucasian |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001016 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
| Human Race: | Hispanic and Caucasian |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| RB14 B05 | SA059448 | FL010499 | Hispanic | Ethnicity |
| RB14 B05 | SA059448 | FL010499 | Male | Gender |
| RB14 B05 | SA059448 | FL010499 | NAFLD | Group |
Collection:
| Collection ID: | CO001010 |
| Collection Summary: | Blood was collected as a part of a routine/pre-operation check up, not more than 2 weeks prior to operation day (bariatric surgery) for the NAFLD group and among normal BMI healthy volunteers for the control group. All volunteers were fasted 10-12 hours before collection. |
| Sample Type: | Blood (plasma) |
| Collection Method: | Venipuncture |
| Storage Conditions: | -80℃ |
| Collection Vials: | EDTA Vacutainer tubes |
| Collection Tube Temp: | Ambient |
| Additives: | EDTA |
Treatment:
| Treatment ID: | TR001030 |
| Treatment Summary: | Subjects were divided into two groups either: Healthy control or Nonalcoholic fatty liver disease (NAFLD) |
Sample Preparation:
| Sampleprep ID: | SP001023 |
| Sampleprep Summary: | After sample randomization, 50 µl plasma aliquots were enriched with deuterated surrogates in 10 µL methanol (Table S1 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 200 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol:acetonitrile was added. Protein precipitate and debris were removed by centrifugation for 10 min at 4,500 RCF. The supernatant was filtered by centrifugation through 0.2 µm PVDF membranes (Agilent Technologies, Santa Clara, CA, USA) at 500 RCF for 5 min. |
| Sampleprep Protocol Filename: | V_Medici_Plasma_Oxy-EC_analysis_protocol.pdf |
Combined analysis:
| Analysis ID | AN001598 | AN001599 | AN001600 | AN001601 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
| Units | Concentration (pmol/gram) | Concentration (pmol/gram) | % | % |
Chromatography:
| Chromatography ID: | CH001123 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 60 °C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 mL/min |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 µL |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 20 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 µL |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 µL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001476 |
| Analysis ID: | AN001598 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Concentration (pmol/gram) |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001477 |
| Analysis ID: | AN001599 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Concentration (pmol/gram) |
| Ion Mode: | POSITIVE |
| MS ID: | MS001478 |
| Analysis ID: | AN001600 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | % |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001479 |
| Analysis ID: | AN001601 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | % |
| Ion Mode: | POSITIVE |
