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MB Sample ID: SA060050
| Local Sample ID: | 4 |
| Subject ID: | SU001021 |
| Subject Type: | Fish |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001021 |
| Subject Type: | Fish |
| Subject Species: | Megalobrama amblycephala |
| Taxonomy ID: | 75352 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 4 | SA060050 | FL010577 | Control group | Diet |
Collection:
| Collection ID: | CO001015 |
| Collection Summary: | After 16 weeks of the feeding trial, approximately 24h after the last feeding, fish in all tanks were anaesthetized with MS-222 (Sigma Aldrich, USA) at the concentration of 100 mg L-1. Fifteen specimens from each tank were selected randomly to obtain the blood. Blood was obtained from the caudal vein using sterile syringes with pre-added anticoagulant solution and then centrifuged (3000×g) at 4°C for 10 min to obtain the serum, which was quickly frozen in liquid nitrogen and stored at -80°C for biochemical assays. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001035 |
| Treatment Summary: | Control diet (CD group) was formulated to contain 27.11±0.38% carbohydrates. The high-carbohydrate diet (HCD group) was formulated to contain over 36.75±0.92% of easily digestible carbohydrates (mostly from flour). The betaine treatment diet (HCB) to contain a high level of carbohydrates (35.64±0.43%) with 1% betaine supplemented for 16 weeks. HC4B group was fed a combination of HCD (first twelve weeks) and HCB diets (last four weeks). |
| Treatment Protocol Filename: | Experiment_methods.docx |
Sample Preparation:
| Sampleprep ID: | SP001028 |
| Sampleprep Summary: | Fifty μl of protein precipitant (containing NVL) was added to 50 μl of serum solution of each sample, mixed thoroughly, and centrifuged at 13200 rpm for 4 min. The supernatant (10 μl) was mixed with 50 μl methanol borate buffer (0.1 M, pH=8.8) thoroughly. For amino acids, the above solution was mixed with 20 μl of diethyl ethoxymethylene-malonate at 55°C for 15 min. |
Combined analysis:
| Analysis ID | AN001608 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Unspecified |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex API 3200 QTrap |
| Ion Mode | POSITIVE |
| Units | umol/L or ng/mL |
Chromatography:
| Chromatography ID: | CH001130 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Unspecified |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001486 |
| Analysis ID: | AN001608 |
| Instrument Name: | ABI Sciex API 3200 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
