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MB Sample ID: SA064494
| Local Sample ID: | ms6808 - 15 |
| Subject ID: | SU001065 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001065 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| ms6808 - 15 | SA064494 | FL011002 | Nucleus fraction | sample type |
| ms6808 - 15 | SA064494 | FL011002 | IV aKG | Grouping |
Collection:
| Collection ID: | CO001059 |
| Collection Summary: | DIPG IV and DIPG XVII cell lines are collected in this experiment. Susupension cells are harvested using centrifugation at 1200 rpm for 5 min. 1 mL of the supernatant media werr collected in an eppendorf tube and snapped frozen. The cell pellets were broken up into single cell suspension and counted. 1 million cells were taken from the stock and washed 1 x with PBS using table top centrifuge with 10 sec quick spin. The resulting cell pellet was snap frozen. Both the frozen media and cell pellet are stored in -80 C prior transfer. |
| Sample Type: | Glioma cells |
Treatment:
| Treatment ID: | TR001079 |
| Treatment Summary: | Glutamine is required for survival in H3K27M cell lines. DIPG cell lines with the H3K27M mutation were grown in regular media (45mM glucose + 4.5 mM glutamine), glutamine (Gln) free media, and Gln free media with alpha-ketoglutarate (α-KG). Study Design Factors IV REG / GF / aKG = DIPG IV celline grown in regular media / glutamine free media / glutamine free meida with alpha-ketoglutarate XVII REG / GF / aKG = DIPG XVII celline grown in regular media / glutamine free media / glutamine free meida with alpha-ketoglutarate |
Sample Preparation:
| Sampleprep ID: | SP001072 |
| Sampleprep Summary: | TCA Concentrations in glioma cell lines separated into nucleus and mitochondrial fractions. |
Combined analysis:
| Analysis ID | AN001683 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5977A |
| Ion Mode | POSITIVE |
| Units | nmol/vial |
Chromatography:
| Chromatography ID: | CH001184 |
| Instrument Name: | Agilent 7890B |
| Column Name: | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001558 |
| Analysis ID: | AN001683 |
| Instrument Name: | Agilent 5977A |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
