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MB Sample ID: SA070410
| Local Sample ID: | 12_Caki-1_adPGC1A_13CGlc_rep2-cells-polar_GCMS_A |
| Subject ID: | SU001084 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001084 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 12_Caki-1_adPGC1A_13CGlc_rep2-cells-polar_GCMS_A | SA070410 | FL011171 | Caki-1 | cell_type |
| 12_Caki-1_adPGC1A_13CGlc_rep2-cells-polar_GCMS_A | SA070410 | FL011171 | adPGC1A_13CGlc | protocol.id |
Collection:
| Collection ID: | CO001078 |
| Collection Summary: | Collection and quench of cells in tissue culture Media is extracted from plate. |
| Collection Protocol ID: | cell_quench_collection cells_media |
| Collection Protocol Filename: | WS_Fan_Quench_cell_tissue.pdf |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001098 |
| Treatment Summary: | Grown in unlabeled glucose and infected with adGFP. Grown in labeled glucose and infected with adGFP. Grown in unlabeled glucose and infected with adPGC1A. Grown in labeled glucose and infected with adPGC1A. |
| Treatment Protocol ID: | adGFP_12CGlc adGFP_13CGlc adPGC1A_12CGlc adPGC1A_13CGlc |
Sample Preparation:
| Sampleprep ID: | SP001091 |
| Sampleprep Summary: | Lipid extraction from cells. Polar extraction from cells. Protein extraction and quantification. |
| Sampleprep Protocol ID: | lipid_extraction polar_extraction protein_extraction |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf WS_Fan_Prot_Quant.pdf |
Combined analysis:
| Analysis ID | AN001708 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | GC-EI-MS, Thermo ITQ |
| Column | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| MS Type | EI |
| MS instrument type | GC-ITQ |
| MS instrument name | Thermo ITQ |
| Ion Mode | POSITIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001205 |
| Chromatography Summary: | Targeted GC |
| Instrument Name: | GC-EI-MS, Thermo ITQ |
| Column Name: | SGE 0.15 mm i.d. x 50 m fused silica open-tubular column, coated with 0.25 μm BPX-5 (5% phenylmethyl- phenylsiloxane) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001581 |
| Analysis ID: | AN001708 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivitization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide). |
| Ion Mode: | POSITIVE |
