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MB Sample ID: SA071498
| Local Sample ID: | MAP317 |
| Subject ID: | SU001096 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 50-90 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001096 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 50-90 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MAP317 | SA071498 | FL011211 | Diffuse abdominal/intestinal pain | Abdominal pain location (symptom) |
| MAP317 | SA071498 | FL011211 | Intestinal Mechanical Obstruction | clinical diagnosis |
Collection:
| Collection ID: | CO001090 |
| Collection Summary: | Blood was drawn at admission to the emergency room, after acquiring the appropriate informed consent (explaining to the patients the aims of the study and ensuring them the complete anonymity of the data collected). Each sample consisted of 8-10 cc of peripheral blood and was immediately ultra-centrifuged for about 10-15 minutes at 1300 RCF. Serum samples were then subdivided in small aliquots (about 500-600 microliters each), and immediately stored at -80 °C. |
| Collection Protocol Filename: | Blood_collection.docx |
| Sample Type: | Blood (serum) |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR001110 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP001103 |
| Sampleprep Summary: | Frozen serum samples were thawed at room temperature and 300 μl of each sample were added to a total of 300 μl of a sodium phosphate buffer at pH 7.4 (75 mM Na2HPO4, 20 % (v/v) D2O, 0.025 % (v/v) NaN3 and 0.8 % (w/v) sodium trimethylsilyl[2,2,3,3-22H4]propionate (TMSP)). From the mixture, 450 μl were transferred into a 4.25 mm NMR disposable tube, acquired from Bruker BioSpin srl. |
| Sampleprep Protocol Filename: | NMR_Sample_preparation.docx |
| Processing Storage Conditions: | Room temperature |
Analysis:
| MB Sample ID: | SA071498 |
| Analysis ID: | AN001723 |
| Analysis Type: | NMR |
| Analysis Protocol File: | NMR_details.docx |
| Data Format: | 1r |
| Num Factors: | 6 |
NMR:
| NMR ID: | NM000129 |
| Analysis ID: | AN001723 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 600 MHz |
| NMR Solvent: | Serum D2O Buffer |
| NMR Tube Size: | 4.25 mm |
| Temperature: | 36.85 |
| Number Of Scans: | 64 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.7 |
| Relaxation Delay: | 4 |
| Line Broadening: | 1 Hz |
| Chemical Shift Ref Std: | TMSP at 0 ppm |
| Binned Increment: | 0.02 |
| Binned Data Excluded Range: | 4.40-5.00 (ppm) H2O region |
