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MB Sample ID: SA072633
| Local Sample ID: | MQ_33 |
| Subject ID: | SU001122 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
| Age Or Age Range: | 45-50 days |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
| Animal Light Cycle: | 12:12 h |
| Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001122 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
| Age Or Age Range: | 45-50 days |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
| Animal Light Cycle: | 12:12 h |
| Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
| Animal Water: | ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MQ_33 | SA072633 | FL011382 | WT | Genotype |
Collection:
| Collection ID: | CO001116 |
| Collection Summary: | Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used. |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR001136 |
| Treatment Summary: | The animals did not receive any treatment |
Sample Preparation:
| Sampleprep ID: | SP001129 |
| Sampleprep Summary: | Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform). |
| Sampleprep Protocol Filename: | NMR_quadriceps_sample_prep_protocol. pdf |
Analysis:
| MB Sample ID: | SA072633 |
| Analysis ID: | AN001760 |
| Analysis Type: | NMR |
| Analysis Protocol File: | NMR_data_acquisition_method.pdf |
| Processing Parameters File: | Data_processing_method.pdf |
| Num Factors: | 2 |
| Num Metabolites: | 36 |
| Units: | Normalized peak area |
NMR:
| NMR ID: | NM000131 |
| Analysis ID: | AN001760 |
| Instrument Name: | Bruker Avance III HD |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Spectrometer Frequency: | 500 MHz |
