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MB Sample ID: SA074537
| Local Sample ID: | sample.5 |
| Subject ID: | SU001138 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001138 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| sample.5 | SA074537 | FL011447 | 2PAHs | Treatment |
| sample.5 | SA074537 | FL011447 | 30 min | Time |
Collection:
| Collection ID: | CO001132 |
| Collection Summary: | For the global metabolomics with a lipid focus, immediately following treatment, cell monolayers were washed 3 times with cold PBS, and cells were scraped and fixed in 500 μL 70% MeOH. Samples were stored immediately at -80°C until pretreatment for solid phase extraction (SPE). |
| Collection Protocol Filename: | AKB_Untargeted-protocol.docx |
| Collection Protocol Comments: | For AKB study 1 protocol, the collection, treatment, and sample prep are all in the same file. |
| Sample Type: | Epithelial cells |
Treatment:
| Treatment ID: | TR001152 |
| Treatment Summary: | For the global metabolomics with a focus on the lipids: Cells (passage <20) were maintained in CMRL 1066 media containing 10% FBS and 1% glutamate in a humidified atmosphere at 37˚ C, 5% CO2, and 95% air (Osgood et al. 2013). Once cells were grown to confluence, cells were serum deprived for 24 hr prior to treatment with the binary 1:1 LMW PAH mixture of 1-MeA and Flthn at a final dose of 40 μM. This mixture has been used previously in our lab (PMC5808746) to represent two LMW PAH’s common in secondhand smoke and environmental exposures. |
| Treatment Protocol Filename: | AKB_Untargeted-protocol.docx |
| Treatment Protocol Comments: | For AKB study 1 protocol, the collection, treatment, and sample prep are all in the same file. |
Sample Preparation:
| Sampleprep ID: | SP001145 |
| Sampleprep Summary: | The hydrophobic fraction was extracted from the samples using an organic liquid-liquid extraction technique with methyl tert-butyl ether (MTBE) and water (Cruickshank-Quinn et al. 2014; Yang et al. 2013). The upper hydrophobic layer was transferred to a new tube. The hydrophobic layer was then dried under nitrogen, reconstituted in 200 µL of methanol, and stored at -80 °C until LCMS analysis. |
| Sampleprep Protocol Filename: | AKB_Targeted-protocol.docx |
| Sampleprep Protocol Comments: | For AKB study 1 protocol, the collection, treatment, and sample prep are all in the same file. |
Combined analysis:
| Analysis ID | AN001780 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Agilent Zorbax Rapid Resolution HD SB-C18 |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent 6210 TOF |
| Ion Mode | POSITIVE |
| Units | abundance/counts |
Chromatography:
| Chromatography ID: | CH001258 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent Zorbax Rapid Resolution HD SB-C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001644 |
| Analysis ID: | AN001780 |
| Instrument Name: | Agilent 6210 TOF |
| Instrument Type: | TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
