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MB Sample ID: SA077759
| Local Sample ID: | S00015469 |
| Subject ID: | SU001178 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001178 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S00015469 | SA077759 | FL011827 | Tissues | Sample type |
| S00015469 | SA077759 | FL011827 | Mus musculus | Species |
| S00015469 | SA077759 | FL011827 | N | bleomycin/IPF |
| S00015469 | SA077759 | FL011827 | Y | saline/HLF |
| S00015469 | SA077759 | FL011827 | Y | wt B6 |
Collection:
| Collection ID: | CO001172 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001193 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP001186 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A010-Ceramides.pdf |
Combined analysis:
| Analysis ID | AN001834 | AN001835 | AN001836 | AN001837 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Phenyl | Phenyl | HILIC | GC |
| Chromatography system | Agilent | Agilent | Agilent | Agilent GC_7890N |
| Column | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| MS Type | ESI | ESI | ESI | EI |
| MS instrument type | Triple quadrupole | Triple quadrupole | QTOF | Single quadrupole |
| MS instrument name | Agilent 6490A QQQ | Agilent 6490A QQQ | Agilent 6520B QTOF | Agilent 5975 |
| Ion Mode | POSITIVE | POSITIVE | NEGATIVE | POSITIVE |
| Units | counts | uM | uM | uM |
Chromatography:
| Chromatography ID: | CH001312 |
| Instrument Name: | Agilent |
| Column Name: | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) |
| Column Temperature: | 40 |
| Flow Rate: | 200ul/min |
| Solvent A: | 100% water; 5 mM ammonium acetate,pH 9.9 |
| Solvent B: | 60% acetonitrile/40% isopropanol |
| Chromatography Type: | Phenyl |
| Chromatography ID: | CH001313 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001314 |
| Instrument Name: | Agilent GC_7890N |
| Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001695 |
| Analysis ID: | AN001834 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | QM033_2mmC18_Ceramide_MRM |
| Analysis Protocol File: | A010_Ceramides.pdf |
| MS ID: | MS001696 |
| Analysis ID: | AN001835 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | QM033_2mmC18_Ceramide_MRM |
| Analysis Protocol File: | A010_Ceramides.pdf |
| MS ID: | MS001697 |
| Analysis ID: | AN001836 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m |
| Analysis Protocol File: | A011_Glycolysis-TCA-nucleotides.pdf |
| MS ID: | MS001698 |
| Analysis ID: | AN001837 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | ALPHA KETO ACIDS-FULL.M |
| Analysis Protocol File: | A011_Glycolysis-TCA-nucleotides.pdf |
