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MB Sample ID: SA078297
| Local Sample ID: | 6_MDA231_13C15NGln_shP4HA1_rep1-cells-polar_NMR_A |
| Subject ID: | SU001203 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001203 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 6_MDA231_13C15NGln_shP4HA1_rep1-cells-polar_NMR_A | SA078297 | FL011942 | 13C15NGln_shP4HA1 | protocol.id |
Collection:
| Collection ID: | CO001197 |
| Collection Summary: | Collection and quench of cells in culture |
| Collection Protocol ID: | cell_quench_collection |
| Collection Protocol Filename: | 3A_Cells_Quench_Cell_Fan_20170412.pdf |
| Sample Type: | cultured cells |
Treatment:
| Treatment ID: | TR001218 |
| Treatment Summary: | Cells grown in unlabeled glutamine and infected with empty vector. Cells grown in unlabeled glutamine and infected with shP4HA1 lentivirus. Cells grown in labeled glutamine and infected with empty vector. Cells grown in labeled glutamine and infected with shP4HA1 lentivirus. |
| Treatment Protocol ID: | 12C14NGln_shCTL 12C14NGln_shP4HA1 13C15NGln_shCTL 13C15NGln_shP4HA1 |
Sample Preparation:
| Sampleprep ID: | SP001211 |
| Sampleprep Summary: | Polar extraction from cells. Protein extraction and quantification. |
| Sampleprep Protocol ID: | polar_extraction protein_extraction |
| Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D_17Jun4_Fan_Prot_Quant.pdf |
Analysis:
| MB Sample ID: | SA078297 |
| Analysis ID: | AN001869 |
| Analysis Type: | NMR |
| Num Factors: | 2 |
| Num Metabolites: | 22 |
| Units: | normalized peak area |
NMR:
| NMR ID: | NM000147 |
| Analysis ID: | AN001869 |
| Instrument Name: | Agilent 600 |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | HC-HSQC |
| Standard Concentration: | 0.5 mM |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | cryogenic triple resonance HCN |
| NMR Solvent: | D20 |
| NMR Tube Size: | 1.7 mm |
| Shimming Method: | gradient shimming |
| Pulse Sequence: | gHSQCAD |
| Water Suppression: | presaturation |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 15 celsius |
| Acquisition Time: | 2 s |
| Relaxation Delay: | 4 s |
| Baseline Correction Method: | Bernstein Polynomial |
