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MB Sample ID: SA079877
| Local Sample ID: | FFS108B |
| Subject ID: | SU001216 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19 - 26 yr |
| Weight Or Weight Range: | 50.6 - 94.1 kg |
| Height Or Height Range: | 1.6 - 1.85 m |
| Gender: | Male and female |
| Human Smoking Status: | Non-smoker |
| Human Exclusion Criteria: | Subjects with anemia, diabetes, thyroid disease, MetS, cancer, previous cardiovascular events or other disease diagnoses were excluded. Subjects were also excluded if they had extreme dietary or exercise patterns, or were taking prescription medications or other supplements known to alter lipoprotein metabolism such as isoflavones. |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001216 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19 - 26 yr |
| Weight Or Weight Range: | 50.6 - 94.1 kg |
| Height Or Height Range: | 1.6 - 1.85 m |
| Gender: | Male and female |
| Human Smoking Status: | Non-smoker |
| Human Exclusion Criteria: | Subjects with anemia, diabetes, thyroid disease, MetS, cancer, previous cardiovascular events or other disease diagnoses were excluded. Subjects were also excluded if they had extreme dietary or exercise patterns, or were taking prescription medications or other supplements known to alter lipoprotein metabolism such as isoflavones. |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| FFS108B | SA079877 | FL012242 | FF | Treatment |
| FFS108B | SA079877 | FL012242 | Post | Timepoint |
Collection:
| Collection ID: | CO001210 |
| Collection Summary: | HDL fractions were isolated from plasma using a 2-step sequential density-based ultracentrifugation method |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001231 |
| Treatment Summary: | Each subject was given either a fast food or a Mediterranean for 4 days in randomized order. |
Sample Preparation:
| Sampleprep ID: | SP001224 |
| Sampleprep Summary: | Lipids were extracted using methanol-MTBE method. |
| Sampleprep Protocol Filename: | zivkovic_protocol.pdf |
Combined analysis:
| Analysis ID | AN001899 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE |
| Units | ug/ml |
Chromatography:
| Chromatography ID: | CH001375 |
| Methods Filename: | zivkovic_protocol.pdf |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65°C |
| Flow Gradient: | 0 min 15% (B), 0–2 min 30% (B), 2–2.5 min 48% (B), 2.5–11 min 82% (B), 11–11.5 min 99% (B), 11.5–12 min 99% (B), 12–12.1 min 15% (B), 12.1–15 min 15% (B) |
| Flow Rate: | 0.6 mL/min |
| Injection Temperature: | 4°C |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001755 |
| Analysis ID: | AN001899 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Data are analyzed in a four-stage process. First, raw data are processed in an untargeted (qualitative) manner by Agilent’s software MassHunter Qual to find peaks in up to 300 chromatograms. Peak features are then imported into MassProfilerProfessional for peak alignments to seek which peaks are present in multiple chromatograms, using exclusion criteria by the minimum percentage of chromatograms in which these peaks are positively detected. We usually use 30% as minimum criterion. In a tedious manual process, these peaks are then collated and constrained into a MassHunter quantification method on the accurate mass precursor ion level, using the MS/MS information and the LipidBlast library to identify lipids with manual confirmation of adduct ions and spectral scoring accuracy. MassHunter enables back-filling of quantifications for peaks that were missed in the primary peak finding process, hence yielding data sets without missing values. |
| Ion Mode: | NEGATIVE |
