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MB Sample ID: SA080342
| Local Sample ID: | JHOP-05731 |
| Subject ID: | SU001223 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001223 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| JHOP-05731 | SA080342 | FL012312 | Feces | Sample |
| JHOP-05731 | SA080342 | FL012312 | Angiotensin II | Treatment |
Collection:
| Collection ID: | CO001217 |
| Collection Summary: | Feces |
| Collection Protocol Filename: | Sample_Collection-Feces_Pluznick.pdf |
| Sample Type: | Feces |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001238 |
| Treatment Summary: | Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. |
| Treatment Protocol Filename: | Treatment_Protocol__Pluznick.pdf |
Sample Preparation:
| Sampleprep ID: | SP001231 |
| Sampleprep Summary: | Samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |
| Sampleprep Protocol Filename: | Sample_Preparation_Pluznick.pdf |
Combined analysis:
| Analysis ID | AN001916 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer |
| Column | C18 |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE |
| Units | Peaks area |
Chromatography:
| Chromatography ID: | CH001389 |
| Methods Filename: | Ultrahigh_Performance_LC_Method_Pluznick.pdf |
| Instrument Name: | Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer |
| Column Name: | C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001772 |
| Analysis ID: | AN001916 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | None |
| Ion Mode: | NEGATIVE |
