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MB Sample ID: SA082256

Local Sample ID:160627_Smp_6R1
Subject ID:SU001252
Subject Type:Other
Subject Species:Marine Plankton

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Subject:

Subject ID:SU001252
Subject Type:Other
Subject Species:Marine Plankton

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
160627_Smp_6R1SA082256FL0124791Replicate

Collection:

Collection ID:CO001246
Collection Summary:Cultured marine plankton cells grown to mid-to-late exponential phase were filtered onto 0.2-micron filters and extracted for metabolites as described in methods. Environmental plankton samples were collected onto 0.2-micron filters during a diel study (Diel_study_StationCastNumber) in the North Pacific.
Sample Type:Plankton cells

Treatment:

Treatment ID:TR001267
Treatment Summary:Marine plankton cells were cultured according to standard protocols, and environmental samples were collected in situ.

Sample Preparation:

Sampleprep ID:SP001260
Sampleprep Summary:Marine plankton cells were extracted using the described protocols.
Sampleprep Protocol Filename:SP_Ingalls_extraction_protocol.pdf

Combined analysis:

Analysis ID AN001970 AN001971 AN001972 AN001973 AN001974
Analysis type MS MS MS MS MS
Chromatography type HILIC HILIC HILIC HILIC HILIC
Chromatography system Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class Waters Acquity I-Class
Column SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um) SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
MS Type ESI ESI ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Orbitrap Orbitrap Orbitrap
MS instrument name Waters Xevo-TQ-S Waters Xevo-TQ-S Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE NEGATIVE NEGATIVE NEGATIVE POSITIVE
Units nanomolar in extraction vial picomoles per liter of filtered seawater peak area per liter of filtered seawater peak area Peak area

Chromatography:

Chromatography ID:CH001425
Methods Filename:CH_Ingalls_LC_protocol.pdf
Instrument Name:Waters Acquity I-Class
Column Name:SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Column Temperature:30
Flow Rate:0.15 mL/min
Solvent A:85% acetonitrile/15% water; 10 mM ammonium carbonate
Solvent B:40% acetonitrile/60% water; 10 mM ammonium carbonate
Chromatography Type:HILIC

MS:

MS ID:MS001824
Analysis ID:AN001970
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol.
Ion Mode:NEGATIVE
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
  
MS ID:MS001825
Analysis ID:AN001971
Instrument Name:Waters Xevo-TQ-S
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:See protocol.
Ion Mode:NEGATIVE
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
  
MS ID:MS001826
Analysis ID:AN001972
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol.
Ion Mode:NEGATIVE
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
  
MS ID:MS001827
Analysis ID:AN001973
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol.
Ion Mode:NEGATIVE
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
  
MS ID:MS001828
Analysis ID:AN001974
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See protocol.
Ion Mode:POSITIVE
Analysis Protocol File:MS_Ingalls_MS_protocol.pdf
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