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MB Sample ID: SA086155
Local Sample ID: | wt3 |
Subject ID: | SU001285 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 24 weeks |
Weight Or Weight Range: | 20-30 gram |
Gender: | Male |
Animal Housing: | Research Animal Resource Center of Weill Cornell Medicine |
Animal Feed: | regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan) |
Animal Water: | regular water |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001285 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 24 weeks |
Weight Or Weight Range: | 20-30 gram |
Gender: | Male |
Animal Housing: | Research Animal Resource Center of Weill Cornell Medicine |
Animal Feed: | regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan) |
Animal Water: | regular water |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
wt3 | SA086155 | FL012849 | Control-chow | Treatment |
Collection:
Collection ID: | CO001279 |
Collection Summary: | Pieces of organ tissue were snap-frozen in liquid nitrogen |
Sample Type: | Kidney |
Treatment:
Treatment ID: | TR001300 |
Treatment Summary: | After weaning for 2 weeks all mice were randomized to a Vitamin A (VA) sufficient regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan). Mice were sacrificed by cervical dislocation after 24 weeks to harvest the organs. |
Treatment: | vitamin A deficient diet (#88407, Harlan) |
Treatment Compound: | chow diet |
Treatment Route: | oral |
Treatment Dose: | ad libitum |
Treatment Doseduration: | 24 weeks |
Treatment Vehicle: | none |
Animal Vet Treatments: | none |
Animal Fasting: | Mice were fasted overnight prior to sacrifice |
Sample Preparation:
Sampleprep ID: | SP001293 |
Sampleprep Summary: | After washes in cold PBS, samples were lysed by three cycles of bead-beating in 80% -70 ᵒC methanol: water using a tissue lyser cell disrupter. Proteins were removed from the extraction mixture by centrifugation to yield the metabolites for analysis. The metabolites were dried in a speed-vac, and stored at -80 ᵒC. Prior to measurement, the metabolites were solubilized in 0.2 M NaOH and subsequently analyzed by LC/MS and LC MS/MS |
Combined analysis:
Analysis ID | AN002031 |
---|---|
Analysis type | MS |
Chromatography type | Normal phase |
Chromatography system | Agilent 6550 |
Column | Cogent Diamond Hydride HPLCs,MicroSolv Technology Corporation |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6550 QTOF |
Ion Mode | UNSPECIFIED |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001472 |
Chromatography Summary: | Aqueous Normal Phase |
Instrument Name: | Agilent 6550 |
Column Name: | Cogent Diamond Hydride HPLCs,MicroSolv Technology Corporation |
Column Temperature: | 25 |
Flow Gradient: | linear |
Flow Rate: | 0.4ml/min |
Solvent A: | 50% isopropanol/50% water |
Solvent B: | 90% acetonitrile/10% water |
Chromatography Type: | Normal phase |
MS:
MS ID: | MS001884 |
Analysis ID: | AN002031 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MS only molecular feature extraction Agilent MassProfinder 8.0 |
Ion Mode: | UNSPECIFIED |