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MB Sample ID: SA086158
| Local Sample ID: | wt1 |
| Subject ID: | SU001285 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 24 weeks |
| Weight Or Weight Range: | 20-30 gram |
| Gender: | Male |
| Animal Housing: | Research Animal Resource Center of Weill Cornell Medicine |
| Animal Feed: | regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan) |
| Animal Water: | regular water |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001285 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 24 weeks |
| Weight Or Weight Range: | 20-30 gram |
| Gender: | Male |
| Animal Housing: | Research Animal Resource Center of Weill Cornell Medicine |
| Animal Feed: | regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan) |
| Animal Water: | regular water |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| wt1 | SA086158 | FL012849 | Control-chow | Treatment |
Collection:
| Collection ID: | CO001279 |
| Collection Summary: | Pieces of organ tissue were snap-frozen in liquid nitrogen |
| Sample Type: | Kidney |
Treatment:
| Treatment ID: | TR001300 |
| Treatment Summary: | After weaning for 2 weeks all mice were randomized to a Vitamin A (VA) sufficient regular chow (#88406, Harlan, Madison, WI) or VA-deficient diet (#88407, Harlan). Mice were sacrificed by cervical dislocation after 24 weeks to harvest the organs. |
| Treatment: | vitamin A deficient diet (#88407, Harlan) |
| Treatment Compound: | chow diet |
| Treatment Route: | oral |
| Treatment Dose: | ad libitum |
| Treatment Doseduration: | 24 weeks |
| Treatment Vehicle: | none |
| Animal Vet Treatments: | none |
| Animal Fasting: | Mice were fasted overnight prior to sacrifice |
Sample Preparation:
| Sampleprep ID: | SP001293 |
| Sampleprep Summary: | After washes in cold PBS, samples were lysed by three cycles of bead-beating in 80% -70 ᵒC methanol: water using a tissue lyser cell disrupter. Proteins were removed from the extraction mixture by centrifugation to yield the metabolites for analysis. The metabolites were dried in a speed-vac, and stored at -80 ᵒC. Prior to measurement, the metabolites were solubilized in 0.2 M NaOH and subsequently analyzed by LC/MS and LC MS/MS |
Combined analysis:
| Analysis ID | AN002031 |
|---|---|
| Analysis type | MS |
| Chromatography type | Normal phase |
| Chromatography system | Agilent 6550 |
| Column | Cogent Diamond Hydride HPLCs,MicroSolv Technology Corporation |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6550 QTOF |
| Ion Mode | UNSPECIFIED |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001472 |
| Chromatography Summary: | Aqueous Normal Phase |
| Instrument Name: | Agilent 6550 |
| Column Name: | Cogent Diamond Hydride HPLCs,MicroSolv Technology Corporation |
| Column Temperature: | 25 |
| Flow Gradient: | linear |
| Flow Rate: | 0.4ml/min |
| Solvent A: | 50% isopropanol/50% water |
| Solvent B: | 90% acetonitrile/10% water |
| Chromatography Type: | Normal phase |
MS:
| MS ID: | MS001884 |
| Analysis ID: | AN002031 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS only molecular feature extraction Agilent MassProfinder 8.0 |
| Ion Mode: | UNSPECIFIED |
