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MB Sample ID: SA086876

Local Sample ID:S00031733
Subject ID:SU001292
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001292
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
S00031733SA086876FL012876BaselineTime Point

Collection:

Collection ID:CO001286
Collection Summary:Colonic mucosal tissue
Sample Type:Colon

Treatment:

Treatment ID:TR001307
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP001300
Sampleprep Summary:Two-step solvent extraction. Supernatants are combined, dried, and re-suspended for LCMS separation by RPLC and measurements by ESI- QQQ MRM methods. Analytes are reported as uM, and CV's are generally <15%.
Sampleprep Protocol Filename:Bile_acids_analysis.doc

Combined analysis:

Analysis ID AN002038
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters Acquity BEH C18 (50 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units pmol/mg

Chromatography:

Chromatography ID:CH001478
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-I.m
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C18 (50 x 2mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001890
Analysis ID:AN002038
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NA
Ion Mode:POSITIVE
Acquisition Parameters File:2014-07-30-Bile_acid-full-BEH-C18-I.m.zip
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